JAWAHAR ATANUVASVIJAYARANI KSARATHCHANDRA GPAZHANIVEL NMANOHARAN S2023-12-292023-12-292022https://krishikosh.egranth.ac.in/handle/1/5810205064Bovine tuberculosis is one of the major disease affecting humans, bovines and wildlife, by virtue of its economic importance and public health significance. For early diagnosis of the disease, initial attempts were made to genomically characterize the Mycobacterium tuberculosis complex (MTBC) organisms causing disease in bovine or wildlife and to prepare a diagnostic reagent, purified protein derivative (PPD) from one of the more recent strains involved in the disease condition. To ensure adequate quantity and quality of DNA extracted from nasal secretions of animals, cotton or tampon swabs were tested. It was found that tampon swabs could extract more nasal secretion leading to significantly higher DNA yields and quality. The sensitivity of the use of tampon swab in detecting known culture was ten times higher than cotton swabs. The excretory pattern of MTBC and Non tuberculousmycobacteria (NTM) from tuberculin skin test positive animals was erratic and inconsistent. The samples of nasal secretions, milk and lymph node aspirates resulted only in isolation of NTMs over MTBC organisms. The NTM were found to grow abundantly and early as compared to M. bovis isolates. They could probably mask the growth of MTBC leading to preferential isolation of NTMs from bovine excretions especially when the animals are maintained in an environment rich in NTM organisms.EnglishThesis