RamneekKaur, Manpreet2017-05-262017-05-262015-09-24http://krishikosh.egranth.ac.in/handle/1/5810012629Sub clinical mastitis (SCM) in buffalo is considered as multi-factorial disease, which leads to huge economic losses by reducing the quality and quantity of the milk. Although bacteria are considered to be the main causative agent for mastitis, farm management practices, genetic make-up of the animal are found to be associated with the disease. So the present study aimed at identification of SNPs in the bovine CXCR1 through PCR-SSCP, DNA sequencing and its association if any with mastitis tolerance/succeptibility in Indian water buffalo... Animal wise the prevalence of sub clinical mastitis was found to be (27. %), and 7.9%, 9.9%, 2.9% quarters were found to be affected with latent mastitis, specific mastitis and nonspecific mastitis respectively . On the basis of individual quarter, the highest prevalence was in left hind (33.5%), followed by right hind (29.7%) and right front (22.2%) and the least prevalence in left front (14.6%). The microorganisms isolated from sub clinical mastitis constituted Staphylococci (39%), Streptococci (31%), Corynebacterium (25%) and E.coli. (5%).PCR-SSCP when used to explore polymorphism in partial exon 2 of CXCR1 gene, the 311bp fragment of this gene was found to be monomorphic in all the DNA samples screened. SSCP analysis of the exonic amplicons and DNA sequence analysis of CXCR1 gene exonic sequences (using one set of PCR primers) did not reveal any polymorphism in this region of CXCR1 gene in diseased or healthy buffaloes. Farm management associated determinants (type of feed, type of floor, education of the owner, feeding after milking, type of labor) when studied in association with sub clinical mastitis, education of the owner , type of labor and feeding after milking were found to be significantly associated with disease occurrence (p<0.05). While looking at the animal determinants in association with sub clinical mastitis in the present investigation, buffaloes with higher parity showed more risk of developing sub clinical mastitis. So the present investigation helped to understand the various risk factors for the occurrence of sub clinical mastitis in buffaloes in Punjab.ennullThesis