V. Sagar, SharathRavi Latha, BhaskaranSangaran, A, et al.,TANUVAS2022-05-102022-05-102021-12https://krishikosh.egranth.ac.in/handle/1/5810184555TNV_JVP_2021_35(2)120-125with cell density, aerobic conditions and the toxic effect of metabolite produced post induction. In the current study, the ideal expression conditions needed to obtain optimum yield of a putative chitinase from Rhipicephalus sanguineus sensu lato using recombinant Rosetta (DE3)pLysS cells was evaluated. The expressed proteins were visualised in SDS-PAGE and confirmed by western blotting. Double selection of colonies expressing the recombinant protein was carried in the study. The study showed that induction at an OD600 range of 0.6-0.8 and aerobic conditions with the induction volume being one-fifth the flask volume favoured the expression of recombinant protein. Post-induction incubation periods of 1- 16hr did not influence protein production among the induced cultures.EnglishVeterinary ScienceArticle