Reena, DLakshmi, GKurunchi Malar, SManokaran, STANUVAS2021-12-062021-12-062021https://krishikosh.egranth.ac.in/handle/1/5810178676TNV_IJCMAS_2021_10(3)1860-1866The study was conducted to explore the cryoprotective effect of L-proline on sheep oocyte vitrification. In vitro matured sheep oocytes (n=836) were vitrified by open pulled straw method in vitrification solution consisting of 15 per cent (v/v) DMSO+ 15 per cent (v/v) EG + sucrose (0.5M) + 20 per cent FBS in TCMh (Group I) and 7.5 per cent (v/v) DMSO + 10 per cent (v/v) EG + sucrose (0.5M) + 20 per cent FBS in TCMh supplemented with 2M L-proline (Group II). The oocytes were warmed and observed for viability by tryphan blue staining. The mean ± SE live oocyte percentage was significantly higher in group II than in group I (59.80±1.02 vs. 44.99±1.34). The vitrified thawed oocytes were activated parthenogenitically using ionomycin and 6-DMAP and developmental stages of embryos were analysed. In parthenogenetically activated embryos, there was no significant difference was observed in cleavage rate (47.56±2.09 vs/ 42.45±3.44) and morula (14.90±1.68 vs. 12.95±2.25) percentage between L-proline and control group. From the study it is concluded that L-proline could be used as a cryoprotectant with high efficiency in sheep oocyte vitrification.EnglishVeterinary ScienceArticle