Dogra, SheetalGupta, Vrinda2023-11-132023-11-132023-10-18Gupta, V. 2023. In vitro Propagation Studies in Rose (Rosa hybrida L.)37081https://krishikosh.egranth.ac.in/handle/1/5810201083The present investigation entitled “In vitro propagation studies in rose (Rosa hybrida L.) was conducted at Tissue Culture Lab, Udheywalla, SKUAST- Jammu during the year 2022-23. Two explants i.e., axillary buds and shoot tips were used for the study. MS medium supplemented with BAP (1, 2 and 3mg/l), Kn (1 and 2 mg/l) and GA3 (1and 2mg/l) were employed for shoot regeneration. MS basal medium + 2mg/l BAP + 2mg/l GA3 was found superior for maximum survival of explants (69.67%), earliest shoot emergence (6.00 days), highest number of shoots per explants (2.50) and maximum shoot length (2.50 cm) and was at par with MS medium + 1mg/l BAP + 2mg/l GA3. Axillary buds were found to be suitable for culture establishment, since it gave maximum survival (53.67), earliest shoot emergence (9.90), highest number of shoots per explants (1.86) and maximum shoot length (1.74 cm). Interaction between explants and shoot regeneration media was significant except for number of shoots per culture. This medium was further used for multiplication of regenerated shoots. For rooting, half and full strength MS medium with IBA (1, 1.5 and 2mg/l) were used. Half MS medium with IBA (1.0mg/l) was found superior in earliest root emergence (14.67 days) and per cent rooting (74.33). However, number of roots per shoot (8.07) and root length (4.5 cm) were found best with 1/2 MS medium + 2.0mg/L IBA. For biological hardening bio-agents with potting media consisting: cocopeat, perlite and vermiculite were used. Trichoderma viride with Cocopeat 2:Perlite 1 gave highest survival (78.00%) and plant height (11.07 cm). Application of Pseudomonas flourescens with Cocopeat 2:Perlite 1:Vermiculite 1 resulted in maximum number of leaves per plant (9.33) whereas, maximum root length (8.57) was recorded when Trichoderma viride with Cocopeat 2:Perlite 1:Vermiculite 1 was used.EnglishThesis