Tewari, A.K.Dwivedi, Meenakshi2018-10-222018-10-222014-07http://krishikosh.egranth.ac.in/handle/1/5810081878Biocontrol is an important approach for plant disease management under changing food habits a and Trichoderma is an important component of these strategies and Trichoderma spp. can be found in locations that stretch the entire globe in a variety of soils and habitats. They are well recognized as biocontrol agents for soil borne plant pathogens and potential plant growth stimulators. In the present investigation Trichoderma were isolated from the soil samples collected from rhizosphere (RS) and rhizoplane (RP) of wheat (W), rice (R) and chickpea (C) from diseased fields. Based on visual differences in cultural characteristics different Trichoderma isolates isolated from their native crops were selected for further studies. Among 27 isolates (RS-18; RP-9) from wheat, 19 (RS-11; RP-8) were selected, 27 isolates (RS-17; RP-10) from rice, 16 (RS-9; RP-7) were selected and 24 isolates (RS-15; RP-9) from chickpea, 16 (RS-9; RP-7) were selected to study their efficacy on growth promoting effects in their native crops. Based on the performance on growth promoting effects in-vitro and in-vivo in their native crops i.e. wheat, rice and chickpea, 07, 06 and 06 promising Trichoderma isolates, , respectively were selected and further evaluated for their growth promoting effects in their native crops in-vitro and in-vivo. The results showed that 02 Trichoderma isolates from each crop viz. TRP-W8 & TRSW4 (wheat) , TRS-R8 & TRS-R4 (rice) and TRP-C4 & TRP-C3 (chickpea) were found significantly better than other isolates, standard check (Th-14) and check in promoting growth of their native crops. The selected Trichoderma isolates (02 no. from each crop) were further tested for their growth promoting potential on their native crops and vice-versa. In-vitro and in-vivo studies revealed that Trichoderma isolates from their native crops were found significantly superior in germination, plumule/shoot length & weight, radical/root length & weight and seedling/plant vigour index in their native crops as compared to other crops. Among these Trichoderma isolates TRP-W8 (wheat), TRS-R8 (rice) and TRP-C4 (chickpea) were found significantly best in germination and plant vigour index (3865.0 & 9919.68; 4115.0 & 6204.12 and 5303.0 & 5215.68) as compared to check (2807.0 & 7359.0; 3072.0 & 5200.0 and 3190.0 & 4060.3) in vitro and in vivo in their native crop viz. wheat, rice and chickpea, respectively. All the selected Trichoderma isolates (06 no.) were identified as Trichoderma viride (T. asperellum) with the help of Bio-Log system. In dual culture studies, all the 06 Trichoderma isolates (TRP-W8, TRS-W4, TRS-R8, TRSR4, TRP-C4 and TRP-C3) were found very effective in parasitizing (100%) the test pathogens viz. Sclerotinia sclerotiorum (chickpea), Rhizoctonia solani (chickpea and rice) and Bipolaris sorokiniana (wheat) within 7 days after contact. The present investigations revealed that crop native Trichoderma isolates are more efficient in increasing seed germination and plant vigour in their native crops as compared to other crops. Therefore, these crop native Trichoderma isolates could be better exploited in their native crops after testing their antagonistic potential against the plant pathogens for overall crop health and productivity under IDM /organic cultivation for sustainable agriculture.ennullThesis