Gupta, M. P.Seema2019-02-282019-02-282005http://krishikosh.egranth.ac.in/handle/1/5810097578The study was carried out in a herd of 80 animals during period from March 2004 to April 2005. Incidence rate of mastitis was 36.14 per cent. The symptoms varied from inflammatory swelling of mammary gland or fibrosis of mammary gland, secretion of either thicker or thinner consistency of milk, presence of flakes, pus and blood clots in milk. Mean value of pH of milk sample f rom diseased animals ranged from 6.41 ± 0.578 to 8.416 ± 0.622. Mean value of somatic cell count in affected animals ranged from 14.25 ± 9.49 to 35.5 ± 20.07 lac cells/ml of milk and 1.67 ± 1.67 to 6.56 ± 3.12 lac cells/ml of milk during pre treatment and post treatment period respectively. Vitamin C level was found to be decreased in animals suffering form mastitis viz. from 1.0081 ± 0.307 to 1.0215 ± 0.0127 mg/di from normal level of vitamin C 1.096 ± 0.11 to 1.0988 ± 0.0134 mg/di estimated in healthy animal i.e. decreased by 8 per cent. There was significant reduction in vitamin E level during pre treatment period in mastitis affected animal i.e. 3.303 ±0.0384 to 3.445 ± 0.1899 vtrno1/1 from normal level of vitamin E i.e. 4.341 ± 0.216 to 4.365 ± 0.239 estimated in healthy animal i.e. decreased by 28 per cent. There was significant reduction in selenium i.e. 0.303 ± 0.90 to 0.311 ± 0.097 ug/m1 in infected animals from normal healthy animals i.e. 0.751 ± 0.056 to 0.786 ± 0.056 vtg/m1 i.e. decreased by 59 per cent. Vitamin C deficient animals were treated with injection redoxin 20m1 i/m for five days and they showed restoration of normal value. Vitamin E deficient animals were treated with vitamin E capsules, vitamin E care Se injection and injection vetade separately and animal showed improvement and they restored normal value. The recovery rate was 50 per cent, 66.66 per cent, 66.66 per cent, 80.33 per cent and 100 per cent in vitamin C treated, vitamin E capsule and vitamin E care Se injection treated, vetade treated, antibiotic treated and antibiotic in combination with vitamin E care Se injection treated animals respectively. The organisms isolated were mostly Gram -ve bacilli. In some animals Gram +ve cocci were also isolated. Pefloxacin was found the most sensitive drug during culture sensitive test. The samples have been collected from buffaloes suffering from mastitis and were treated similarly successfully.en-usThesis