Jose, T MPatil Chandrakant, SitaramKAU2019-05-312019-05-312001http://krishikosh.egranth.ac.in/handle/1/5810106090PGSex pheromones are reported to be present in many crustaceans which mostly have releaser effect on sexual behaviour. The discovery of a male (primer) pheromone with stimulatory effect on ovarian maturation and ovulation has added an important external factor to the basic mechanisms regulating reproduction in fresh water prawns. But studies in these lines are very few in the economically important species of Macrobrachium. Knowledge about this male pheromone will be useful to help or potentiate reproduction and/or extend the breeding period of freshwater prawns, which are of great aquacuItural importance. The present study was carried out in the slender nver prawn, Macrobrachium idella. Seven ovarian maturity stages have been distinguished here. Histological studies of the maturing oocytes helped to understand the changes taking place during oocyte growth and maturation through the progressive maturity stages. Yolk vesicles start appearing in the cytoplasm of oocyte in maturity stage II which is the beginning of vitellogeneis (vitellogenesis I). In stage IV, yolk platelet synthesis starts and 'a peripheral layer of yolk platelets appear in this stage. Later these yolk platelets completely fill the ooplasm through stages V and VI when the ova increase in size enormously. Gonado- somatic index (ovarian index) of different maturity stages was determined and was found useful to differentiate especially the late maturity stages. Ova diameter studies along with the other observations showed a clear picture of protracted multiple spawning in this species with batches of ova undergoing maturation and ovulation in successive maturation cycles. When the females were reared in isolated condition, their ovarian growth was found to be arrested at early stage IV. This restriction of ovarian maturation was found mostly in the second maturation (gonadal) cycle after isolation. But those isolated females which had a common water circulation with males showed normal ovarian maturation and ovulation. Therefore, it can be concluded that a male pheromone exists in this species which is essential for normal ovarian growth and maturation. Another experiment was conducted to find out the tissue source of this putative male pheromone. When these isolated females were exposed to extracts of testis, vas deferens or muscle, both testis and vas deferens extracts were found to stimulate the ovarian maturation and ovulation in isolated females, while the muscle extract (control) was ineffective. This shows that in M idella, the male pheromone is produced by testis and vas deferens or it is produced by testis and transported to vas deferens. Further experiment showed that charcoal extraction destroys the pheromonal activity in testis and vas deferens extract while boiling has no such effect which indicates that the ovary stimulating pehromone in M idella may be a steroidal molecule as reported in some fishes.ennullThesis