Dudhare, M.S.Jadhav, Akshaykumar Balaji2023-03-212023-03-212023-03-0323271https://krishikosh.egranth.ac.in/handle/1/5810195578Linseed (Linum usitatissimum L.) is a vital oilseed and fibre crop grown for its seed still as fibre which is employed for the manufacture of linen. The oil is edible and even have industrial use like preparation of paints, varnishes, printing ink, soap, oil, cloth, leather, and waterproof fabrics. In the present study the investigation done on linseed morphology and molecular screening for drought tolerance. Under normal conditions among all genotypes earliest flowering was observed in DEEPIKA (41 days). Under stress condition the earliest flowering variety are KARTIKA (41 days) and DEEPIKA (41 Days). Under normal conditions among all lines, the tallest plant was observed in normal condition is RLC-172(55.80cm). Under stress condition the tallest plant was observed is RLC-172 (56.10cm). Under normal conditions the maximum number of fertile capsules observed in RLC-161(24). Under stress condition the maximum number of fertile capsules observed in RLC-143 and RLC- 92(15). Under normal conditions the maximum 1000 seed weight observed in RLC- 167(6.80gm). Under stressed conditions the maximum 1000 seed weight observed in RLC-172(6.15gm). The stressed linseed genotypes showed rise in proline accumulation over unstressed control. Under normal conditions the highest proline content observed in KIRAN (6.62 µmol/g). Under stress condition the proline content the highest proline content observed in KIRAN (18.89 µmol/g). The maximum root length observed in normal condition is KARTIKA(8.6cm). Under stressed condition the root length varies from 3.75cm to 8.96cm with an average of 6.37cm. maximum root length in stressed condition observed in KARTIKA(8.96cm). The present ISSR primers investigation revealed that the mean polymorphic information content (PIC) score for each primer ranged from 0.34 to 0.71 with a mean of 0.55 The least relevant primer was noticed to be ISSR primer UBC-807 of PIC value of 0.34, while the maximum informative marker UBC-825 had a PIC value of 0.71. The similarity matrix of 18 linseed lines varies from 0.20(IA-32-DEEPIKA) to 0.92(RLC-167-RLC-148). Thus, most similar genotypes observed were RLC-167 and RLC-148. The most dissimilar genotypes observed were IA-32 and DEEPIKA were noticed. The dissimilarity matrix of 18 linseed lines varies from 0.08(RLC-167- RLC-148) to 0.77 (IA-32-DEEPIKA). Thus, most dissimilar genotypes observed were IA-32-DEEPIKA. The most dissimilar genotypes observed were RLC-167-RLC-148 were noticed. The present SSR primers investigation revealed that the mean polymorphic information content (PIC) score for each primer ranged from 0.15 to 0.84. The least relevant primer was noticed to be SSR primer LU-9 of PIC value of 0.15, while the maximum informative marker LU-3 had a PIC value of 0.84. Thus, most similar genotypes observed were RLC-92 and RLC-153 and RLC-133. The most dissimilar genotypes observed were IA-32 and KIRAN were noticed. The dissimilarity matrix of 18 linseed lines varies from 0.00(RLC-92-RLC-153) to 0.77 (IA-32-KIRAN). Thus, most dissimilar genotypes observed were IA-32-KIRAN. The most dissimilar genotypes observed were RLC-92 and RLC-153 were noticed.EnglishThesis