Chakraborty, DibyenduAzad, Mandeep Singh2023-01-042023-01-042022-11-09https://krishikosh.egranth.ac.in/handle/1/5810191172Indigenous / native population is very important source of genes for future breeding and improvement purposes. They are more adapted to the local environment and have a natural resistance to some diseases. Therefore, the present study was undertaken with the objectives to characterize the Poonchi chicken population by microsatellite markers and to characterize the immune related genes namely ChB6, IAP-1 and IL-15Rα. A total 50 unrelated blood samples of indigenous chicken from its natural breeding tract were utilized for molecular characterization. Genomic DNA was isolated. Ten (10) FAO recommended microsatellite markers namely ADL0268, MCW0206, MCW0081, ADL0278, MCW069, MCW0248, MCW0111, MCW0222, MCW0016, and LEI0094 were used for molecular characterization. PCR amplifications were performed with some modifications and PCR products were run in 10% Urea-PAGE for genotyping. Microsatellite data were analyzed by using molecular biology software Popgen32. For molecular characterization of immune related genes RNA was isolated and cDNA was synthesized. Primers were designed and PCR amplification was performed. Sequencing of the products was done and results were analyzed by using MEGAX and BioEdit softwares. A total of 72 alleles across 10 different microsatellite loci under present study were observed with mean number of loci as 7.200±1.2293. The Chi-square test revealed deviation from HWE. The mean na, ne and Shannon’s information index (I) in the population were 7.2000, 5.2928 and 1.7648, respectively. The PIC values for different microsatellite loci ranged from 0.6348 (LEI0094) to 0.9751 (MCW0016). The overall average inbreeding coefficient (FIS) was -0.0416. The results obtained from sequence analysis showed that there was no variation in the sequences of Poonchi, Kadaknath and Leghorn chicken populations for ChB6 and IAP-1 genes but there was variation with Fayoumi and other reported Gallus gallus sequences and other species. For IL-15Rα gene there was variation within Poonchi population as well as between different chicken populations like Kadaknath, Leghorn, Fayoumi. From the present study it can be recommended that further studies on large population should be carried out to explore the molecular level variations for formulation of breeding strategies, genetic improvement and conservation of indigenous chicken populationEnglishThesis