Shilpa2017-03-212017-03-212011http://krishikosh.egranth.ac.in/handle/1/5810005586The present study was aimed at profiling and molecular tagging of glucosinolates (leaves and seeds) and tocopherols (seeds) in B. juncea germplasm collection maintained at PAU, Ludhiana. Recombinant inbred line (RIL) population comprising F6 and F7 progenies, (developed by crossing NUDH-YJ-04; low glucosinolate European juncea and RL-1359; high glucosinolate Indian juncea, were evaluated during years 2008-2011 in Oilseeds section, Department of Plant Breeding and Genetics, for biochemical traits viz. oil quality (i.e. tocopherols) and meal quality (i.e. glucosinolates) which were later used for molecular tagging. In the first year, approximately 366 RILs were screened for total glucosinolate content in the seeds and out of these, 97 lines were selected on the basis of range observed for total glucosinolate content. In these 97 lines, values for total glucosinolates in seeds and leaves, total tocopherol and oil content in seeds ranged from 15.73-127.61 (µmole/g DW seed), 4.33-129.93 (µmole/g DW leaf), 32.29-1250 (ppm) and 34.96-45.00 (%) respectively. Individual components (GNA, SIN, IBE, NAS and NEO) in seeds and (GNA, SIN and EPIPRO) in leaves, obtained from profiling ranged from (2.36-90.87 µmole/g DW seed), (0.194-35.16 µmole/g DW seed), (0.117-10.93 µmole/g DW seed), (0.0-6.10 µmole/g DW seed), (0.0-0.99 µmole/g DW seed) and (0.653-73.04 µmole/g DW leaf), (0.089-4.85 µmole/g DW leaf) and (0.65-16.37 µmole/g DW leaf) respectively. Biochemical data generated from these evaluations was used for molecular tagging. SSR markers (414) were evaluated on parents and out of these, 100 markers were found to be polymorphic which were then screened on 92 RILs. 100 polymorphic markers gave 106 loci, out of which 82 were found to be linked. A framework linkage map of 1718.9 cM length with 20 linkage groups was developed from 82 linked loci. From this linkage map, a QTL map has been developed which gave four QTLs with total glucosinolates as significant QTL (LRS-14.5, R2-15%) and GNA (LRS-11.2, R2-11%), NAS (LRS-12.2, R2-13%) and total tocopherol (LRS-11.0, R2-11%) as the suggestive QTLs. Total glucosinolates and total tocopherols were found to be NUDH-YJ-04 specific alleles and GNA and NAS as RL-1359 specific alleles. More number of significant QTLs with higher R2 values can be obtained by using higher number of polymorphic markers which will generate a highly saturated linkage map. In future, our study can act as a skeleton for tagging and MAS of economically important traits.ennullThesis