B.R. PatilAshwini Narasimhan2016-11-112016-11-112011http://krishikosh.egranth.ac.in/handle/1/85197The present investigation was carried out with an objective to characterize the NBS-LRR degenerate markers in urdbean and mungbean and thus to study the polymorphism at RGA loci. Thirteen and twelve genotypes of mungbean and urdbean were screened for MYMV, powdery mildew and CLS diseases. The genetic variability was also studied. Similarly, genetic variability and molecular diversity was studied in the germplasm lines of mungbean and urdbean in three different experiments. None of the mungbean genotypes were found resistant to all the three diseases while Uttara was found resistant to all the three diseases among the urdbean genotypes. RGA1-TG and MtB99 were specific to MYMV. Selection based on number of pods per plant, number of branches and bunches and total seed yield with high GCV and PCV is effective. Very high heritability and genetic advance estimates were recorded for all the characters studied among mungbean, unlike in urdbean, where high heritability with high genetic advance estimates were recorded for plant height, number of branches, number of bunches, number of pods per plant and total seed yield indicating the role of additive gene action. Seed yield was found to have non significant association with all the characters studied among the urdbean genotypes. The genotype TAU7 formed a separate cluster from the rest of the thirty nine genotypes making it an obvious choice in breeding program. Among the seventy nine germplasm lines of mungbean seed yield per plant was significantly and positively associated with number of pods per plant, number of seeds per pod and test weight. The genotype Uttara can be used as one of the parents in resistance breeding progamme. The two putative markers identified can be used to differentiate the resistant and susceptible genotypes among mungbean genotypes and they could also be further validated using mapping populations.Genetics and Plant BreedingThesis