Mir, HidayatullahAzam, Khushboo2017-07-122017-07-122017-07http://krishikosh.egranth.ac.in/handle/1/5810025053The present investigation entitled “Molecular characterisation and its association with the horticultural traits of elite mango cultivars” was conducted at All India Coordinated Research Project (AICRP) orchard, sabour, Bhagalpur and in the laboratories of department of PBG and department of Horticulture (Fruits). Twenty mango genotypes were studied for their flowering behavior, fruit quality traits and characterized using 16 SSR markers. The cultivar Langra showed maximum flowering intensity, number of hermaphrodite flowers and number of flowers per panicle. The result revealed that cultivar Fazli showed maximum fruit weight, length and breadth among these twenty cultivars. Biochemical attribute such as ascorbic acid, total sugar, reducing sugar was found maximum in Langra. Molecular characterisation of twenty mango cultivars with 16 SSRs primers resulted in total 58 number of amplified fragments. The number of alleles detected varied from 2 (MIAC 5, MiIIHR12, MiIIHR20a, MiSHRS37) to 6 (MIAC11, MiIIHR20a, mMiCIR027).The allele size varied from 50 bp to 1000bp. PIC value ranged from 0.05 to 0.38. The dendrogram generated by UPGMA cluster method placed twenty mango cultivars in two major clusters. Cluster 1, consisted of 3 cultivars namely Langra, Vanraj and Himsagar. Cluster II consists of rest of the cultivars. Cultivar Fazli and Neelum were found to be distinct from other cultivars. There was comparatively good association recorded with the primers and the horticultural traits. All the 16 polymorphic markers with their alleles were subjected to marker trait association with all the 16 phenotypic traits. Step wise linear regression method was followed to study the marker-trait association. The markers having significant association with the economic traits varied from one (fruit volume, titratable acidity, ascorbic acid, reducing sugars, total sugars and total number of flowers) to eight (number of hermaphrodite flowers). However, since the number of genotypes used in this study as well as the number of polymorphic markers used for screening was less, further validation of these markers and their association with traits mentioned herein is needed in larger germplasm or a mapping population.ennullThesis