Patil, C.R.Parvathi S.2020-02-282020-02-282016-01http://krishikosh.egranth.ac.in/handle/1/5810143845Present study was conducted to develop liquid formulations of Pseudomonas striata, a phosphate solubilizing bacteria using different concentrations of additives, adjuvants, and surfactants. Out of the 32 liquid formulations developed, 12 formulations with population more than 5 x 1010 CFU ml-1, after 84 hr of growth were selected for shelf life studies. Formulation 18 with 10 mM glycerol, 0.5% PEG, 0.05% CMC, 0.15% GA and 250 ppm polysorbate 20, recorded the highest population of 3 x 1010 CFU ml-1 after six months of incubation and was used to optimize the dosage of liquid formulation required to treat maize and sorghum seeds. Results clearly indicated that for treating bold seeds such as maize only 2 ml kg-1 of seeds of this formulation showed higher inoculant survival on seeds (log10 6.72), seed germination (94 %), root length (20.59 cm), shoot length (17.92 cm) and seedling vigour (3620.43). Similarly, for treating small sized seeds such as sorghum 4 ml kg-1 seed of formulation was found optimal. It resulted in higher inoculant survival on seed (log10 5.36), root length (26.60 cm), shoot length (26.03 cm) and seedling vigour (489.33). However, the highest per cent germination (92.5%) of sorghum seeds was observed in seeds treated @ 6 ml kg-1 of this inoculum. The cost involved in developing a litre of the best liquid formulation was ` 149. While the cost of lignite based formulation was ` 60 kg-1 which normally is inoculated @ 20 g kg-1 seed, thus costing about ` 1.2 kg-1. However, our results indicated that only 2 to 4 ml of the liquid formulation was sufficient to treat one kg of seed which costed less than one rupee per kg of seeds, there by reducing the total cost of treating with biofertilizer by 50 - 75%.ennullThesis