Manpreet KaurVaishali2019-11-162019-11-162019http://krishikosh.egranth.ac.in/handle/1/5810135496In the present study, superoxide dismutase mimics activity of pristine and cetyl trimethyl ammonium bromide (CTAB) coated cobalt oxide nanoparticles (NPs) and their graphene oxide (GO) nanocomposite (NC) was compared. The NPs, GO and NCs were synthesized employing sol-gel precursor, modified Hummers method and sonication method respectively. The synthesized NPs, NC were characterized using various techniques viz. SEM, XRD, FT-IR spectroscopy, BET analysis and TEM. CTAB coated cobalt oxide NPs showing highest activity was used for synthesis of NC with GO. Co3O4-GO NC with 1:2 displayed maximum activities among the synthesized NC, pristine GO and Co3O4 NPs. Enzyme mimic activity was evaluated using Marklund and Marklund method. Optimum pH, temperature, substrate concentration, NPs dose were 8.0, 25°C, 10 mM, and 2 mg respectively. Contact time studies were carried out for 3 min using pyrogallol as substrate. Kinetics studies of syntheised NPs showing best activity were conducted using Michelis Menton eqaution. The calculated Km and Vmax value of the NC were 0.021 mM and 0.0884 μmol sec-1 respectively. Thus NC can be further explored for replacing dismutase for varied applications.ennullThesis