Dr. B. B. BhanderiVatalia Dip J.2020-06-262020-06-262018http://krishikosh.egranth.ac.in/handle/1/5810148006Mastitis is one of the costliest diseases of dairy cattle. Mastitis is classified as subclinical and clinical mastitis. Clinical mastitis is a condition, which is characterized by local symptoms like swelling of the udder, heat and pain or systemic symptoms like fever, anorexia, depression with milk abnormalities like milk clots, flakes, watery secretions, blood in milk. About 150 species of microorganisms have been incriminated as the causal agents of mastitis. Some times in clinical mastitis after treatment there are reoccurrence of clinical mastitis. Looking to the economic important the present study was undertaken with the following objectives viz. cultural isolation, biochemical characterization and PCR based identification of bacterial pathogens, extraction of DNA from clinical mastitic milk and PCR based identification of major bacterial pathogens, post therapy isolation, identification and quantitation of bacteria by Real-Time PCR and antimicrobial susceptibility testing of the bacterial isolatesen-USnullThesis