ANITHA PETERVALEETA MARINA DSOUZA2020-10-062020-10-062019-08-22Th-12397https://krishikosh.egranth.ac.in/handle/1/5810152461Coleus forskohlii is a medicinal plant of perennial and aromatic nature. Cancer is one of the leading causes of morbidity and mortality worldwide and the presently available treatments for cancer have several serious side effects and costly. Bio pharming has a great role in producing drugs at low cost. In the present investigation TNF Related Apoptosis Inducing Ligand (TRAIL) gene was made to expressed in the Coleus forskohlii. The standardization of regeneration protocol of Coleus forskohlii using leaf explants was conducted. Various concentrations of growth hormones such as auxin (IAA) and cytokinin (BAP) were tired to induce regeneration. Significantly highest percent of callus, mean number of shoots per callus and the rooting was obtained in the combination containing 9 mg L-1 of BAP and 0.4 mg L-1 of IAA. Screening of plant putative transformants was done using PCR for gene integration and for the expression studies, c-DNA analysis, direct plate ELISA, indirect dot immuonobinding assay (DIBA) and SDS-PAGE analysis was carried out. PCR amplification showed the presence of 400bp of TRAIL gene amplicon in transformed plants and the protein size obtained through SDS-PAGE analysis was about 30kDa.EnglishThesis