Veerabramhaiah, K (Major)Mouli Krishna, KVeena, PVENKATESWARA REDDY, Ch2017-06-102017-06-102013-08http://krishikosh.egranth.ac.in/handle/1/5810019308ABSTRACT : Superovulaory response with different mode of administration of FSHp was studied in Sahiwal cows based on estrus response, number of ovulations, embryos recovery and anovulated follicles including endocrine response. All the selected donor cows were synchronized with double PG prior to superovulation and on 3rd day of superovulation with single PG. The difference in the interval between PG treatment and onset of estrus and duration of estrus between the cows synchronized prior to and after superovulation with 250 mg of FSHp in multiple doses (group I) and single dose (group II) was significant (P<0.05). Higher percentage of cows showed intense estrus in group I cows and intermediate estrus in group II cows. Superovulation of group I donor cows resulted in development of 8.50 ± 0.56 and 5.67 ± 0.56 mean number of follicles and mean number of ovulations, respectively and the same in group II donor cows were 5.17 ± 0.17 and 3.33 ± 0.21, respectively. The difference in the total follicles and total ovulations were significantly (P<0.05) higher in group Icows than in group II 12 cows. The overall total mean number of anovulations were significantly higher in group I (2.83 ± 0.17) compared to group II (1.83 ± 0.31). The overall mean number of embryos, transferable embryos and unfertilized ova recovered from group I donor cows were 3.17 ± 0.65, 2.33 ± 0.42 and 0.83 ± 0.31, respectively per collection with an overall mean recovery percentage of 55.88 ± 7.68. The corresponding values in group II donor cows were 1.67 ± 0.21, 1.00 ± 0.26 and 0.67 ± 0.21, per collection with an overall mean recovery percentage of 50.00 ± 6.09. The total number of embryos and transferable embryos recovered after superovulation were insignificantly (P>0.05) higher in group I cows than in group II cows. Embryos collected were cryopreserved by OPS vitrification method. In Group I and II, the overall mean progesterone concentrations recorded on different days of synchronization and superovulation i.e., at 2nd PGF2α, synchronized estrus, initiation of superovulation, PGF2α on 3rd day of superovulation, super estrus and embryo collection were 5.08 ± 0.83, 2.24 ± 0.22,5.24 ± 0.53, 5.12 ± 0.53, 1.93 ± 0.60 and 6.53 ± 0.41 ng/ml, respectively and 3.98 ± 0.83, 2.47 ± 0.22,5.39 ± 0.53, 4.31 ± 0.53, 2.49 ± 0.51 and 4.80 ± 0.56 ng/ml, respectively. The overall mean estrogen concentrations on same days were 15.05 ± 0.55, 26.30 ± 1.20, 17.46 ± 0.54, 32.16 ± 2.41, 39.04 ± 1.06 and 17.72 ± 0.45 pg/ml, respectively in group I and 20.20 ± 0.99, 24.14 ± 0.86, 21.76 ± 0.74, 32.41 ± 2.08, 38.22 ± 0.86 and 20.05 ± 1.03 pg/ml, respectively in group II. The concentrations of progesterone and estrogen at the time of embryo collection were significantly (P<0.05) different between Group I and II. But the difference between the groups at 2nd PGF2α administration and synchronized estrus and after administration of PGF2α, at the time initiation of superovulation, PGF2α administration, super estrus and embryo collection was not significant (P>0.05). There was a positive and significant (P<0.05) correlation between concentrations of progesterone at synchronized estrus and super estrus in group I while a negative and significant (P<0.05) correlation between 2nd PG and anovlations;initiation of superovulation and embryo collection and superovulation PG and embryo collection in group II. The concentration of estrogen at 2nd PG with recovered embryos and transferable embryos and at initiation of superovulation with anovulations was positively and significantly (P<0.05) correlated in Group I and at 2nd PG with recovered embryos and 13 transferable embryos and at initiation of superovulation with transferable embryos was negatively and significantly (P<0.05) correlated.ennullThesis