Kumari, SachinGoel, Neha2022-08-222022-08-222021-09https://krishikosh.egranth.ac.in/handle/1/5810186511The present study was undertaken for studies on phytochemicals and antioxidant potential of Argemone mexicana (Mexican poppy) collected from semi arid region of Haryana. Physiological and chemical parameters viz. moisture, ash, crude fibre, crude fat, crude protein, minerals (Fe, Cu, Zn, Mn), tannins, terpenoids and alkaloids were analyzed in stem, leaves and flowers of Argemone mexicana. Moisture content was higher in stem (13.53%) than leaves (11.06%) and flowers (11.33%). Ash content was maximum in flowers (21.56%) than stem (11.15%) and leaves (14.8%). Crude protein was higher in leaves (23.63%) than stem (15.26%) and flowers (22.32%). Crude fibre content was higher in stem (25.2%) than leaves (9.85%) and flowers (11.75%), crude fat content was higher in flowers (16.9%) than stem (11.56%) and leaves (7.93%). Amount of Fe, Cu, Zn and Mn (in ppm) in flowers was 117.53, 30.60, 14.89 and 21.17 and in the stem, it was 63.61, 7.97, 3.41 and 37.41 and in leaves, it was 93.40, 23.3, 15.71 and 19.58. Total tannins were higher in leaves (3.15mg/g) than stem (1.81mg/g) and flowers (2.41mg/g).). Total alkaloids were higher in leaves (3.97%) than stem (3.23%) and flowers (3.46%). Terpenoids were higher in leaves (3.65%) than stem (3%) and flowers (3.03%). Phytochemical parameters viz. total phenolics and total flavonoids in stem, leaves and flowers were extracted with five solvents namely methanol, aqueous, acetone, ethyl acetate and hexaane. Aqueous extract has the highest phenolic content (8.16mg GAE/g) as compared to methanol extract (7.09mg GAE/g), acetone (2.16mg GAE./g), ethyl acetate (1.36mg GAE/g) and hexane (0.23mg GAE/g). Among the plant, parts flowers have the highest phenolic content (5.75mg GAE/g) than stem (1.86mg GAE/g) and leaves (3.79mg GAE/g). Methanol extract has the highest flavonoid content (6.08mg CE/g) as compared to acetone extract (4.19mg CE/g), aqueous (4.45mg CE./g), ethyl acetate (1.77mg CE/g) and hexane (1.31mg CE/g). Among the plant parts, flowers have the highest flavonoid content (4.68mg CE/g) than stem (2.41mg CE/g) and leaves (3.59mg CE/g). DPPH free radical scavenging activity (%) increased with increase of concentration levels the IC50 value of stem in aqueous, methanol, ethyl acetate, hexane and acetone extract was 58.88, 48.70, 48.02, 41.81 and 24.98μg/ml. IC50 value of leaves in these extracts was 37.28, 45.95, 68.91, 36.29 and 62.29 μg/ml. IC50 value of flowers in these extracts was 41.92, 55.18, 42.37, 45.74 and 38.33μg/ml. Aqueous extract has the highest total antioxidant activity (6.94mgAAE/g) than methanol extract (6.46mgAAE/g), acetone extract (2.32mgAAE/g), ethyl acetate (1.87mgAAE/g) and hexane extract (0.26mgAAE/g). Among plant parts flowers has the highest antioxidant activity (4.19mgAAE/g) than stem (2.66mgAAE/g) and leaves (3.86mgAAE/g).EnglishThesis