Rana, Y.SArya, Kanchan2024-02-202024-02-202023-04https://krishikosh.egranth.ac.in/handle/1/5810206949The occurrence of hepatic lipidosis in 40 dairy Murrah buffaloes at 3 weeks prepartum at CIRB, Hisar using ultrasonography and blood BHB (>0.6 mmol/l) was found to be 22.5 per cent out. Among them, 66.66 per cent and 33.33 per cent of buffaloes were found to be affected with mild and moderate hepatic lipidosis, respectively. The occurrence of hepatic lipidosis in 38 dairy buffaloes at 3 weeks prepartum at Hi-Tech Dairy farm, Sirsa based upon blood BHB (>0.6 mmol/l) was found to be 39.47 per cent. Overall, the erythrocytic indices did not differ significantly in affected animals except for lymphocytopenia. However, significantly lower levels of Hb, PCV and MCHC were found in dairy buffaloes affected with moderate hepatic lipidosis. Also, the affected animals showed relative lymphopenia. Blood BHB was found significantly higher in animals with hepatic lipidosis at 3 weeks prepartum as compared to healthy buffaloes and correlated with the degree of hepatic lipidosis. Serum biochemistry in affected animals revealed hypoglycemia, hypocholesterolemia, lowered triglycerides, hypoproteinemia, hypoglobinemia and elevated ALP levels. Serum minerals and electrolytes profile in affected buffaloes revealed a significantly lower level of total Ca, Cu, and Zn along with hyponatremia, hypokalemia, and hypochloremia. Serum iCa was found significantly low in dairy buffaloes affected with moderate hepatic lipidosis. With respect to oxidative stress indices and acute phase proteins levels, no significant changes were observed in dairy buffaloes with hepatic lipidosis. Serum concentrations of AST were significantly reduced in the ascorbic acid, L-carnitine and silymarin group compared to the positive control group at 2 weeks postpartum. Total protein levels differ significantly at 6 weeks postpartum in the group supplemented with ascorbic acid and L –carnitine as compared to the control group. The cholesterol level was significantly higher towards 6 weeks postpartum in the group supplemented with ascorbic acid. The creatinine level was decreased in the L- carnitine group as compared to the control group at 6 weeks postpartum. The iCa level significantly increased at 2 and 6 weeks postpartum in groups supplemented with ascorbic acid, L- carnitine and Silymarin as compared to the positive control. No significant difference was found in the acute phase proteins with respect to therapy within the group however silymarin has a lower level of Haptoglobin 6 weeks postpartum as compared to other groups. No significant changes were observed in oxidative stress indices with respect to therapy. Overall, a positive response was recorded in all the treated groups; however, the groups treated with L-carnitine and Silymarin showed better ameliorative efficacy.EnglishThesis