Muthuraju, RMuthuraju, RNALINI, B.SNALINI, B.S2023-01-052023-01-052022-12-172022-12-17Th-12782Th-12782https://krishikosh.egranth.ac.in/handle/1/5810191263A study was conducted to isolate, screen of actinobacterial isolates for the growth promotional, biocontrol efficiency and evaluation of efficient isolates on growth and yield of cowpea and ragi under greenhouse conditions. Forty actinobacterial isolates were isolated. Biochemical characterization of actinobacterial isolates showed 2 isolates are positive for casein hydrolysis, 11 isolates for tyrosine, 20 isolates for H2S production, eight and seven isolates could produce melanin and iterate, respectively and negative for HCN and urease production. Among them, 26 isolates produced IAA and GA. UASBA46 and UASBA50 isolates showed he highest P, K and Zn solubilization with positive for siderophore, ammonia production. Highest concentration of ACC-deaminase activity and also production f hydrolytic enzymes observed in both the isolates. In vitro studies showed that 15 isolates were antagonistic against fungal pathogens Sclerotium Rolfson and Pythium spp 13 isolates were antagonists against Fusarium oxysporum. Further, actinobacterial isolates were identified as Streptomyces antibioticus UASBA50) and Streptomyces sp. (UASBA46) by 16s rRNA partial genome sequencing. Significant increase in plant growth and yield parameters was bserved in the treatment receiving dual inoculation of actinobacterial isolates with RDF in cowpea and finger millet. Significant increase in soil enzymes activity and total microbial population was also recorded. Jaggrey solution with NaCl+MgSO4+CaSO4 found to be the best substrate for mass multiplication of actinobacterial isolates. Hence the application of actinobacterial isolates has multiple growth and yield promotional activity as biofertilizers could serve as romising solution for improving soil health in sustainable crop production.A study was conducted to isolate, screen of actinobacterial isolates for the growth promotional, biocontrol efficiency and evaluation of efficient isolates on growth and yield of cowpea and ragi under greenhouse conditions. Forty actinobacterial isolates were isolated. Biochemical characterization of actinobacterial isolates showed 2 isolates are positive for casein hydrolysis, 11 isolates for tyrosine, 20 isolates for H2S production, eight and seven isolates could produce melanin and iterate, respectively and negative for HCN and urease production. Among them, 26 isolates produced IAA and GA. UASBA46 and UASBA50 isolates showed he highest P, K and Zn solubilization with positive for siderophore, ammonia production. Highest concentration of ACC-deaminase activity and also production f hydrolytic enzymes observed in both the isolates. In vitro studies showed that 15 isolates were antagonistic against fungal pathogens Sclerotium Rolfson and Pythium spp 13 isolates were antagonists against Fusarium oxysporum. Further, actinobacterial isolates were identified as Streptomyces antibioticus UASBA50) and Streptomyces sp. (UASBA46) by 16s rRNA partial genome sequencing. Significant increase in plant growth and yield parameters was bserved in the treatment receiving dual inoculation of actinobacterial isolates with RDF in cowpea and finger millet. Significant increase in soil enzymes activity and total microbial population was also recorded. Jaggrey solution with NaCl+MgSO4+CaSO4 found to be the best substrate for mass multiplication of actinobacterial isolates. Hence the application of actinobacterial isolates has multiple growth and yield promotional activity as biofertilizers could serve as romising solution for improving soil health in sustainable crop production.EnglishThesis