SRINIVAS, M(MAJOR)Chandra Prasad, BHari Krishna, N.V.VJAMES FRANCIS, CHRISTO2023-04-162023-04-162022-06https://krishikosh.egranth.ac.in/handle/1/5810196473THESESThe present investigation was conducted to evaluate the efficacy of Ceftriaxone Tazobactam+Normal saline (Group I, n=10), Ceftriaxone Tazobactam+EDTA-Tris (Group II, n=10) and EDTA-Tris (Group III, n=10) in the treatment of subclinical endometritis in postpartum Graded Murrah buffaloes. Buffaloes (n=65) presented with the history of repeat breeding were screened by per rectal examination of the genital tract, followed with assessment of cervico-vaginal mucus score, pH, uterine discharge sample optical density and uterine discharge cytology. Assessment of risk factors responsible for development of endometritis revealed that, pluriparous buffaloes with poor body condition score and maintained under poor housing hygiene had greater risk for occurrence of endometritis. Occurrence of symmetrical uterine horns with normal consistency and thick and heavy uterine horns with extra curvature upon per rectal examination were 72.31 (47/65) and 27.69 per cent (18/65), respectively. The 47 endometritic buffaloes with normal, symmetrical uterine horns were further screened for subclinical endometritis by assessment of cervico-vaginal mucus score, which revealed that 38 (80.85%) buffaloes showed clear mucus discharge of score 0. The pH of cervical mucus was moderately alkaline (7.5 to 8.0) in a high proportion of buffaloes (65.79%) which were suspected of subclinical endometritis. Occurrence of subclinical endometritis (cutoff >0.031 to 0.043) and clinical endometritis (cutoff >0.043) based on optical density measurement at 620 nm was 84.21 and 15.79 per cent, respectively. The results showed that out of the 32 buffaloes evaluated for cytological evidence 93.75% of buffaloes showed ≥5 to 10% and >10 to 15 % PMN cells, while 2 (6.25%) showed >15 per cent PMN cells. Ceftriaxone Tazobactam had shown highest sensitivity (65.0%) upon ABST and it was selected for therapeutic trails by intrauterine route. Biochemical cues revealed that variations in serum and uterine discharges at post-treatment estrus as compared to pre-treatment estrus values indicated that clearing of uterine infection after intrauterine therapy. Higher efficacy for Ceftriaxone Tazobactam+EDTA-Tris (80.00% Vs 87.50%) in terms of recovery and conception rates when compared to treatment with EDTA-Tris (66.67% Vs 71.43%) and Ceftriaxone Tazobactam+Normal saline (60.00 Vs 70.00%). In conclusion, combination of Ceftriaxone Tazobactam with EDTA-Tris had improved the clinical response and conception rate in buffaloes treated for subclinical endometritis.EnglishThesis