GURU VISHNU, P (MAJOR)MURALIDHAR, MANITHA, AREHMAN, SHAIK.2021-07-312021-07-312021-06https://krishikosh.egranth.ac.in/handle/1/5810171252THESESPunganur is a miniature cattle originated from Chittoor district of Andhra Pradesh. Studies so far aimed at characterization of the breed at phenotypic level and molecular level using autosomal microsatellite markers and mitochondrial DNA. In addition to autosomal microsatellite markers and mitochondrial DNA, the Ychromosomal variation also plays a key role in understanding population admixture to trace paternal lineage. Differentiation of paternal lineages via analysis of Ychromosomal variation adds significantly to what can be inferred from mtDNA and autosomal variation in cattle. So far, the Y-chromosomal markers of Punganur cattle is not explored. Hence, the present study is proposed with the following objectives. Objectives of investigation: 1. To characterize Y- chromosome of Punganur cattle using Y chromosome specific markers 2. To determine Y chromosome haplogroups in Punganur cattle A total of 60 Punganur bulls covering different geographical regions in and around Andhra Pradesh and Telangana were screened to identify four Y chromosome specific SNPs (ZFY9:120>C/T; USP9Y: 76426>C/T, SRY: 1748>G/T and UTY19:423>G/T). To detect these SNPs, a PCR-Restriction Fragment Length Polymorphism (RFLP) strategy was designed for screening three SNPs (USP9Y: 76426>C/T, SRY: 1748>G/T and UTY19: 423>G/T) and Allele specific PCR method was adopted for screening SNPs in ZFY9 (120>C/T) in the present study. The resulting restriction profiles and Allele specific genotype were combined to yield haplotype (TCTT) specific for Punganur cattle representing Y3 haplogroup of cattle. These results indicated that all the animals in the present study belong to Y3 haplogroup which underpin the purity of paternal lineage of Punganur cattle. The Y chromosome specific haplotype combination (TCTT) deciphered in our study can be exploited as a marker in Punganur breed conservation and improvement studies for detecting the purity/indigenous origin of paternal lineagesEnglishThesis