Dayal Doss, D.SANTHOSH, D.B.2021-09-282021-09-282018-08-01Th-12072https://krishikosh.egranth.ac.in/handle/1/5810176363Micronutrients malnutrition has reached to alarming situation with billions of people suffering from Iron, Zinc and Vitamin-A deficiencies. Micronutrient supplementation, food fortification and biofortification are the three basic approaches used to alleviate micronutrient deficiencies. In the present study ten genotypes rich in iron were grown during Kharif season 2016 and 2017. The experiment was laid out in Randomized Complete Block Design (RCBD) with three replications. Iron in the aerobic rice grains were estimated by using X-ray fluorescence spectroscopy at M. S. Swaminathan Research Foundation Chennai. Jeerigesanna recorded highest iron content in brown rice (11.75 ppm), paddy (18.77 ppm), 5 per cent bran (22.85 ppm) and 10 per cent bran (25.20 ppm). Whereas, lowest iron content in brown rice was observed in Azucena (7.40 ppm), lowest iron content in paddy was observed in Black rice (10.75 ppm), lowest iron content in 5 per cent bran was observed in AM-65 (12.23 ppm) and lowest iron content in 10 per cent bran was observed in AM-65 (12.35 ppm). Perl’s Prussian blue staining of aerobic rice grain sections showed localization of iron and distribution of iron accumulation (blue compound of ferric ferrocyanide) throughout the aleurone and subaleurone layers. Eleven SSR markers were used three markers RM276, RM315 and RM318 showed polymorphism. Further, Single-marker analysis was done using molecular marker data and phenotypic data of different marker classes revealed that the significant association and linkage of marker to traits like grain iron, panicle length and plant height.EnglishQUANTIFICATION, LOCALIZATION AND MOLECULAR PROFILING OF IRON IN GRAINS OF BIOFORTIFIED AEROBIC RICE (ORYZA SATIVA L.)Thesis