Sinha, M.P.Mahto, Dinesh2017-08-162017-08-162008http://krishikosh.egranth.ac.in/handle/1/5810028708DISPOSITION KINETICS AND EFFICACY OF CEFTRIAXONE SODIUM IN COWS SUFFERING FROM ENDOMETRITISThe present experiment was conducted to study the disposition kinetics and efficacy of ceftriaxone sodium in cows suffering from endometritis belonging to farmer from different farm around Ranchi veterinary college, Kanke, Ranchi. A total of 18 selected cows with endometritis were randomly allocated to 3 groups, comprising of 6 animals in each group. Ceftriaxone sodium at a dose rate of 10 mg/kg body weight was administered only once through I.V., I.M. and I.U. routes to each of 6 animals in different groups and plasma & uterine fluid concentration of the drug was estimated subsequent to drug administration. Efficacy of the treatment of endometritis with ceftriaxone was judged on the basis of bacteriological record and conception rate. The salient features of the present study are summarized below: 1. The average bacterial colonies count recorded was 247.66 X 106 /ml of uterine fluid before treatment in all groups. The mean bacterial colony counts per ml of uterine fluid decreased to the minimum at 3 hours (4.00  0.36 X 106 /ml) after I.V at 1 hours (5.330.55 X 106 /ml) after I.U and at 6 hours (5.330.66 X 106 /ml) after I.M. administration of drug and again increased gradually reaching to 56.66 1.80 X106 /ml, 85.33 2.78 X106 /ml and 81.16  1.24 X106 /ml of uterine fluid , respectively at 72 hours. 2. In-vitro antibiotic susceptibility test of the isolates against ceftriaxone sodium was also done at different levels of drug concentration by Summary &Conclusion 87 tube dilution as well as pour plate test which showed that ceftriaxone was effective at varying concentration and both the method yielding comparable results. 3. The mean plasma concentration (Cp max) of ceftriaxone was recorded at 10 minutes after I.V. (31.15 0.1μg/ml), at 2 hours after I.M. (27.05 1.03μg/ml) and at 4 hours after I.U. (22.98 0.03μg/m) administration. 4. The mean uterine fluid concentration (Cumax) was recorded at 15 minutes after I.U. (157.12  0.71μg/ml), at 2 hours after I.V. (136.87 0.06 μg/ml) and at 6 hours ,after I.M. (132.86  2.26μg/m) administration of the drug. 5. The Cp min after I.V. (1.11 0.80μg/ml) was higher as compared with I.M. (0.79 0.03μg/ml) and I.U. (0.07 0.006μg/ml) administration. 6. The mean Cumin after I.V. (0.79 0.04μg/ml) was marginally higher than I.U. (0.70 0.08μg/m) and after I.M. (0.06 0.007μg/ml) administration. 7. The therapeutic concentration (Cpther, 0.38μg/ml) of the drug in plasma was maintained till 12 hours after single dose I.V. administration, while for 24 hours after I.M. & I.U. administration of drug. 8. The therapeutic concentration of the drug in uterine fluid following I.V. &I.U. administration of the drug was maintained from 15 minutes to 72 hours, while it was maintained between 15 minutes to 48 hours after I.M. administration of the drug. Summary &Conclusion 88 9. The t 1/2 β value after I.U administration (1.93 0.33 hr) was lower than that of I.V (2.59 0.06 hr) and I.M (2.05 0.01 hr) administration. 10. The dosage regimen of Ceftriaxone sodium was calculated to be 8.30 mg/kg to be repeated at 22.24 hours through I.V., 11.33 mg/kg to be repeated at 26.00 hours through I.U. and 9.65 mg/kg to be repeated at 14.33 hours through I.M. for the treatment of endometritic cows. 11. The Ceftriaxone sodium was administered for consecutive 3 days though I.V., I.M. and I.U. routes at a dose level calculated on the basis of pharmacokinetic study in endometritic cows. The conception rates were recorded as 83.33%, 66.67% & 83.33% in cows following I.V., I.M. and I.U. post administration of the drug, respectively.en-USnullThesis