Mishra, D.K.Shahnaz, Kumari2016-07-282016-07-282014http://krishikosh.egranth.ac.in/handle/1/70126The present investigation entitled “Characterization and Quantitative Analysis in Aromatic Rice Germplasm” was carried out at Seed Breeding Farm of J.N.K.V.V, Jabalpur with one hundred and forty nine lines in Randomized Complete Block Design with three replications during Kharif 2013. Morphological observations (as per DUS guideline) viz., basal leaf sheath colour, leaf pubescence of blade surface, leaf auricle, leaf anthocyanin colouration of auricle leaf shape of ligules, leaf ligule colour, flag leaf attitude of blade, stem-anthocyanin colouration of nodes, spikelet colour of stigma, spikelet density of pubescence, sterile lemma colour, spikelet- colour tip of lemma, panicle exsertion, panicle- attitude of branches, panicle- awns, distribution of awns colour of awns were recorded. The quantitative observation were recorded on 10 randomly selected plants e.g. days to fifty percent flowering, plant height, number of tillers plant-1 , number of productive tillers plant-1 , days to maturity, panicle length, average panicle weight per plant, fertile spikelets per panicle, sterile spikelets per panicle, number of spikelets per panicle, spikelet fertility percent, spikelet density, 1000 grain weight, grain yield per plant, biological yield per plant, panicle index, harvest index, and qualitative observation were grain length, grain width, decorticated grain length, decorticated grain width, hulling percent, milling percent. The objective of this study was to estimate genetic variability for yield and quality traits in aromatic rice genotypes, to study genotypic and phenotypic association among all the traits, to estimates direct and indirect effect of yield and quality attributes on seed yield, to indentify superior aromatic rice genotypes based on yield and quality traits and to validate the reported SSR markers on selected genotypes for yield related traits. Characterization of genotypes concluded that the characters viz., basal leaf sheath color, pubescence of blade surface, auricle, auricle color, shape of ligule, ligule color, flag leaf-attitude of blade, spikelet-color of stigma, stem- anthocyanin coloration of nodes, spikelet-density of pubescence, sterile lemma color, spikelet-color of tip of lemma, panicle exsertion, panicle-attitude of branches, awn, distribution of awns, color of awns had sufficient amount of variability. Analysis of variance indicated that the differences among genotypes were highly significant for all the traits studied. This indicated that the genotypes had sufficient amount of variability. The variability among genotypes ranged from number of spikelet per panicle to decorticated grain width. High GCV and PCV exhibited by unfilled spikelet per panicle followed by spikelet no. per panicle, harvest index, grain yield, panicle weight, fertile spikelet no. per panicle, biological yield, spikelet density, productive tillers per plant, total tillers per plant, decorticated grain length, test weight, culm length, panicle index, plant height, spikelet fertility %, grain length, decorticated grain length, grain width, days to 50% flowering, panicle length, days to maturity, hulling % and smallest for milling %. The characters fertile spikelet per panicle, spikelet per panicle, culm length, biological yield, panicle index, spikelet density and test weight exhibited high heritability coupled with high genetic advance. It indicates that the heritability is most likely due to additive gene effect and selection may be effective. Grain yield plant-1 exhibited positive and significant association with panicle weight, Harvest index, Panicle index, Productive tillers per plant, Biological yield per plant, Total tillers per plant, Spikelet fertility %, Fertile spikelet per panicle, test weight, Grain length, Panicle length and Spikelet number per panicle. The highest positive direct effect on seed yield plant-1 was observed for Plant height, spikelet density, harvest index, milling %, biological yield, panicle weight, grain width, plant index, total tillers per plant, test weight, and days to 50% flowering had direct positive effect on grain yield. It indicates true relationship between them and direct selection for these traits will be rewarding for yield improvement. At molecular level, all ten markers used were polymorphic. The total number of alleles amplified was 62 with a mean value 6.20. The highest number of allele were nine amplified by markers RM 276 and RM 223. The highest major allele frequency, gene diversity, heterozygosity and polymorphic information content were 0.6000 (RM 259 and RM 468), 0.8644 (RM 234), 1.0000 (RM 502 and RM 276) and 0.8499 (RM234), respectively. Unique allele and multiple alleles were amplified by four markers RM256, RM276, RM236 and RM234. The cluster analysis on basis of molecular analysis revealed that the selected fifteen genotypes were divided into two major clusters group. Considering, the morphological characterization, quantitative analysis and molecular level it could be concluded that best genotypes are IR 62873-224-1-6 , SUGANDA MATI , IR 62873-227-2-5 , KRISHNA KAMOD , IET 12601 , MUGAD SUGANDHA , IR 62873-244-2-2 , BASMATI MAHON 381 , IR 62873-244-2-5 , UPR 3375-8-1-1 , IR 60164 - 122- 3-2-1 , PUSA BASMATI 6 , IET 12014 and IR 62871-549-3-6.Thus, genotypes might be further used in breeding programmes.enThesis