Dr. M. K. JhalaSAVALIYA, BHUMIKABEN FULABHAI2017-02-232017-02-232015http://krishikosh.egranth.ac.in/handle/1/5810002352The present study was aimed to study rabies virus neutralising antibody status in dogs by ELISA (PLATELIA™ RABIES II ASSAY kit, Bio-Rad) and also comparative evaluation of various rabies virus detection tests. A total of 107 serum samples were collected from vaccinated pet dogs at TVCC, College of Veterinary science and Animal Husbandry, Anand and from Veterinary Poly Clinic, Vadodara and 53 samples from unvaccinated stray dogs at Animal Birth Control Programme (ABC) unit, Ahmedabad to detect rabies virus neutralizing antibodies. The antibody titres obtained by quantitative ELISA were converted into EU/ml as per manufacturer’s instruction by graphpad prism software. Of these, 58 (62.36%) pet dogs and one out of 53 (1.88%) stray dogs showed antibody titre above the cut-off value of 0.5 EU/ml. Vaccinated pet dogs were divided into different groups based on age, sex, breed, vaccine brand and time of vaccination after last vaccine to assess the relationship of these factors with vaccinal immune response. One way analysis of variance (ANOVA) was performed in graphpad prism software to check the effect of all these factors. Statistical analysis of ELISA titres of pet dog serum samples suggested that age, sex, breed, vaccine brands have no significant effect on the antirabies antibody titres. Fourteen non vaccinated pet dog serum samples were checked to detect the maternal antibody status against rabies and of these, only two puppies (aged 2.5 and 2 months) had a protective immune titre above the cut off value of 0.5 EU/ml.enveterinary sciencestudyThesis