Nagesha, S.N.RACHANA D2023-05-112023-05-112023-03-30Th-13617https://krishikosh.egranth.ac.in/handle/1/5810197245SARS-CoV-2 pandemic was reported for the first time at the end of 2019 in the city of Wuhan (China) and has spread worldwide in three years. It has lead to the infection of more than 500 million people and about six million death. SARS-CoV-2 has proved to be very dangerous for human health. The spike protein is a prime target of neutralizing antibodies as it plays critical roles in host cell recognition, fusion, and virus entry and it is composed of two subunits, S1 and S2. The S1 subunit contains the receptor binding domain (RBD) which is involved in the interactions with human angiotensinconverting enzyme-2 (ACE-2) receptor and causes infection leading to the COVID-19 disease. Here, in order to find the target region for developing vaccines we have performed multiple sequence alignment of 157 amino acid sequences of the SARS-CoV-2 spike glycoprotein using BioEdit software and phylogenetic analysis of these sequences were carried out using MEGA-X. Further, multiple sequence alignment of different variants of SARS-CoV2 with reference to the first human SARS-CoV2 virus from Wuhan, China was performed followed by the phylogenetic analysis. In this study it was seen that RBD region(319-541residues) in S1 subunit of Spike protein was conserved having mutation at the 7 positions with sequence identity of 96.30% and this clearly suggests that the S1 subunit (RBD 319-541) can be used as a target region for stable and safe vaccine development.EnglishThesis