R., SIDDARAJUVIJAYALAKSHMI, N.2023-01-042023-01-042021-08-17Th-12921https://krishikosh.egranth.ac.in/handle/1/5810191200Field experiment were conducted to study the effect of spacing and mother plant nutrition on seed yield and quality of parental lines viz., CML-451 (male) and CAL-1443 (female) of hybrid MAH 14-5. The spacing of 30X20 cm with fertilizer level of 225:70:50:10 kg NPK ZnSO4 ha-1 recorded the maximum plant population at harvest in male (127) and female (120) parental lines. Whereas, the highest seed yield was recorded in 30X20 cm with fertilizer of 187:70:50:10 kg NPK ZnSO4 ha-1 (29.43 and 27.48 q/ha) in male and female lines respectively. Seed storage experiment was carried out to know effect of packaging materials, insecticide on quality of hybrid and its parental lines. Germination (86.5 %), seedling vigour index -I (1916), seedling vigour index-II (2738) and seed infestation (1.31 %) was found higher in hybrid MAH 14-5 stored in polypouches treated with chlorantraniliprole @ 0.3 ml/kg seeds at the end of 12th month of storage period. Whereas, least germination (69.25 %), seedling vigour index -I (1916), eedling vigour index-II (2738), and seed infestation (1.31 %) was recorded in female seeds kept as a control in super grain bag. Morphological characterization of hybrid and its parental lines was undertaken. It was observed for pigmentation of brace root, pigmentation of leaf sheath and hairs on leaf margin (absent in female, present in male and slightly present in hybrid), attitude of leaf (female was drooping, straight in male and drooping in hybrid), angle between the main axis and laterals branches (> 450 in female, < 450 in male and in hybrid it was medium angle), tassel (curved in female, straight in male and hybrid). Hybrid purity testing was carried out using 50 SSR markers, out of which eight (phi402893, umc1746, umc1793, umc1232, umc2310, umc2376, umc1308, mmc0151) markers showed the polymorphism differentiating parental lines.EnglishThesis