Ramya, RMadhanmohan, MTANUVAS2022-01-282022-01-282021-04https://krishikosh.egranth.ac.in/handle/1/5810181327TNV_HV_2021_60(SI)34-37Diagnosis of subclinical mastitis is problematic as the milk and udder appears normal but generally has an elevated somatic cell count. In the present study, a new ‘on farm test’ kit “TANUCHEK SCC kit” was evaluated to diagnose subclinical mastitis. Efficiency of this ‘on farm test’ kit was initially compared with California mastitis test (CMT). Further, the results of these indirect tests (TANUCHEK SCC kit and CMT) were compared with the direct test i.e. direct microscopic somatic cell count which was used as reference. Milk samples (n=610) were simultaneously subjected to direct microscopic somatic cell count, CMT and TANUCHEK SCC kit for the presence of subclinical mastitis. The prevalence of subclinical mastitis was 80.33%, 72.95% and 68.85% for the direct test, CMT and TANUCHEK SCC kit, respectively. Kappa statistics was used to compare the results. The TANUCHEK SCC kit had showed substantial agreement (k = 0.8612) and moderate agreement (k=0.5749) with CMT and the reference direct microscopic somatic cell count, respectively. Results of this study revealed that the TANUCHEK SCC kit can be a simple, easily adoptable, on farm screening test to diagnose SCM in field condition.EnglishVeterinary ScienceArticle