Athira, G.R.Chandrasekaran, D.Ramasamy, T.TANUVAS2017-12-282017-12-282017-08http://krishikosh.egranth.ac.in/handle/1/5810038289TNV_CCA_Y2017_3-4Aug.Pg193A 6 year old, HF cross breed cow was brought to the MVC Teaching Hospital with the history of swelling of udder and reduced milk yield. Clinical examination revealed reduced rumen motility, swelling of left forequarter, hot to touch, painful udder, milk was dirty white in colour and milk pH was 8.5. Milk was collected in a sterile culture tube for isolation, identification and bacteriological examination was carried out through growth on blood agar, gram’s staining. Staph. aureus isolates were identified asgram-positive cocci by Gram stain, positive catalase test, growth on mannitol saltagar. Staphylococci was subjected to antimicrobial susceptibility by disc diffusion method to the commonly used antibiotics. Minimum inhibitory concentration was done by modified resazurin assay microdilution technique. MIC (ugm/ml) was 125, 62.5, 125, 125, 65, 250, 0.22, 125, 250 and 250 for azathioprine, gentamicin, tetracycline, oxacillin, cefatoxime, eefuroxime, enrofloxacin, penicillin G, ampicillin and amoxicillin. The isolate was found sensitive for enrofloxacin, gentamicin and cefatoxime and resistant to penicillin G, amoxicillin, ampicillin, azathioprine, tetracycline and cefatoxime. Based on ABST & MIC, cow was treated with enrofloxacin @ 5 mg/kg bwti/m once daily and 100 mg intramammary for 7 days, NSAID meloxicam @ 0.5 mg/kg bwti/m once daily for 5 days, vitamin E @500 IU/day p/o and selenium @ 6 mg / kg p/o for 7 days. PCR amplification of resistance gene was identified.The cow showed clinical improvement after one week of treatment.enVeterinary Science - Conference AbstractOther