Gupta, NamartaHarmanjot Kaur2018-02-012018-02-012017http://krishikosh.egranth.ac.in/handle/1/5810040186In this work, the influence of proline and ascorbic acid on physiological and biochemical parameters in tomato (Solanum lycopersicum L.) seeds of genotypes Punjab Varkha Bahar-4 and Roma at different salinity levels was studied. The salinity stress has deleterious effect on seed germination and seed vigour parameters. Seeds of tomato were treated with different treatments (hydration, different concentrations of proline and ascorbic acid) at 25mM, 50mM and 75mM NaCl concentrations. Various estimates were done from treated seedlings after 14 days of germination. All seed treatments increased the physiological (percent germination, seedling length, seedling biomass, speed of germination, vigour index I and II) and biochemical parameters (total soluble sugars, total soluble proteins and activities of antioxidant enzymes) of seedlings as compared to control at 25mM, 50mM and 75mM NaCl concentrations. The results showed that proline 10mM and ascorbic acid 4mM were more effective than proline 5mM and ascorbic acid 1mM at all salinity levels. Thus, application of proline and ascorbic acid could play an ameliorating role on salt stress tolerance by regulating and scavenging the free radicals produced by reactive oxygen species.ennullThesis