Ahmad, A.H.Sharma, Sonam2020-12-032020-12-032020-10https://krishikosh.egranth.ac.in/handle/1/5810156230Diabetes mellitus is a collection of heterogenous metabolic disorders associated with hyperglycemia as a result of insufficient or no pancreatic insulin secretion or insulin resistance or both. The aim of this study was to assess the antidiabetic potential of hydroethanolic extract of leaves of Vitex negundo (HEVN) following oral administration @ 100mg/kg and 200 mg/kg b.wt. for 28 days in streptozotocin induced diabetic rats. The phytochemical analysis of HEVN revealed 19.28% yield and presence of alkaloids, flavonoids, tannins, terpenoids, saponins, glycosides, phenols and fixed oils and fats. For in-vivo study, forty two rats of 2-2.5 months of age weighing 150-180g were divided randomly in to seven groups of six rats each. Group I served as normal control group. Group II, III, VI and VII, received single intraperitoneal injection of streptozotocin @ 45mg/kg b.wt. to induce diabetes. Rats showing blood glucose level greater than 200mg/dl on 7th day were considered hyperglycemic and chosen for the experiment. Group II represented as diabetic control. Group III (diabetic) was treated with glibenclamide @1mg/kg b.wt. orally for 28days. Group IV and V (non-diabetic) were given HEVN orally @100mg/kg b.wt. and 200mg/kg b.wt., respectively, for 28days. Group VI and VII (diabetic) were given HEVN @100mg/kg b.wt. and 200mg/kg b.wt., respectively, for 28 days. The diabetic rats showed noticeable decrease in body weight which significantly (P<0.05) improved in group VI and VII in a dose dependent manner. Streptozotocin caused significant (P<0.05) elevation in serum ALT, AST, ALP, triglycerides, total cholesterol, BUN and creatinine which were restored back towards normal in HEVN treated groups in a dose dependent manner. A significant (P<0.05) reduction in total protein and albumin was noticed in group II. HEVN administration showed significant (P<0.05) amelioration in the level of total proteins and albumin after 28days in a dose dependent manner. Similarly, HEVN treated rats showed Hb, PCV, TEC and TLC values near to normal control whereas group II showed significant (P<0.05) changes in these parameters as compared to normal control group. On histopathological examination, group II showed histopathological disintegrity in pancreas, liver and kidney which were restored towards normal in HEVN treated rats @200mg/kg b.wt. Thus it is concluded from the present study that hydroethanolic extract of leaves of Vitex negundo (HEVN) @ 100mg/kg and 200mg/kg has ameliorative potential against streptozotocin (@45mg/kg) induced diabetes in rats in a dose dependent manner.EnglishThesis