Sameena MehtabSharma, Shubham2019-07-162019-07-162019-05http://krishikosh.egranth.ac.in/handle/1/5810114899Higher levels of cholesterol lead to life-threatening coronary heart diseases, cerebral thrombosis atherosclerosis etc. therefore monitoring in serum is one of the most important biochemical assay. In the present investigation, non-enzymatic electrochemical sensors was developed for the low level estimation of cholesterol. Polymer nanocomposites have proved to be an excellent transducer in electroanalytical sensing, due to their unique properties like, low cost, good electronic characteristics, rapid electrode kinetics, biocompatibility and environmental stability. The synthesis of polyindole by chemical oxidative polymerization in presence of tungsten carbide ranging from 5 to 15 (%, w/w) and iron (III) chloride as an oxidizing agent. polyindole tungsten carbide electrodes were fabricated and characterized through various spectral, electrical, thermal and electrochemical methods. Synthesized PIN/WC nanocomposites in presence of sulphonated polysulphone was used to modify the surface of stainless steel plate electrode that worked as working electrode in electrochemical studies. The surface morphology of nanocomposites was characterized by SEM. The electrochemical behavior and quantification of cholesterol was studied by cyclic voltammetry and square wave voltammetry in 1 M KOH electrolytic solution. The overall results reveals that developed PIN/WC/SSPE electrode has excellent performance for trace level cholesterol and can be further employed for cholesterol monitoring in blood serum samples.ennullThesis