Vedna KumariSood, Sheetal2019-02-022019-02-022017-07http://krishikosh.egranth.ac.in/handle/1/5810093320The present investigation entitled „Genetic analysis of seed yield and related traits and response to anther culture in brown sarson (Brassica campestris var. brown sarson)‟ was undertaken to assess the nature of genetic variability, extent of genetic diversity and association of various characters with seed yield and their direct and indirect effects under two different environments during rabi 2015-16 and 2016-17. In addition, androgenesis-mediated responsiveness of genotypes and their crosses was also studied through anther culture. Data were recorded on seed yield and various component characters. Reaction to Alternaria blight was studied under natural field conditions at Kangra during rabi 2016-17. The data analysis was done as per standard statistical procedures. The data recorded on anther culture studies were analysed using CPCS software. Sufficient genetic variability was observed for most of the characters in Env.I, Env.II and pooled over the environments. High heritability coupled with high genetic advance was observed for number of secondary branches per plant, seeds per siliqua and harvest index in Env.I, siliquae per plant and number of secondary branches per plant in Env.II and number of primary branches per plant in pooled over the environments which indicated the predominance of additive gene action for the inheritance of these characters. In multivariate analysis, all the genotypes were grouped into six clusters in Env.I, Env.II and pooled over the environments. The clustering pattern of brown sarson genotypes indicated that there was no parallelism between geographical distribution and genetic diversity. Maximum contribution towards genetic divergence was due to seeds per siliqua in Env.I, siliquae per plant in Env.II and days to 75 per cent maturity in pooled over the environments. Maximum genetic divergence was observed between clusters IV and V in Env.I, V and VI in Env.II and II and IV in pooled over the environments which suggested that the hybridisation between genotypes falling in these clusters will give transgressants for high seed yield coupled with earliness. Based upon the correlation and path coefficient analysis, harvest index was observed to be the best selection parameter because of its high, positive direct and indirect contribution towards seed yield per plant. In androgenesis-mediated response, the highest callusing and calli index were observed in N6 medium with HM2 [0.5 mg/l 2, 4-D + 1.0 mg/l NAA] and 3 per cent sucrose. The genotypes HPBS-1 and KBS-3 x HPKM-04-1 performed better in N6 medium for callus induction frequency and calli index, respectively while the genotype HPKM-04-1 appeared to be the best in MS medium for days to calli appearance.ennullThesis