Jain, R.K.Kharb, Anju2016-11-092016-11-092011http://krishikosh.egranth.ac.in/handle/1/84626The present investigation has been undertaken to: (i) analyze the allelic profile of 60 F2 plants obtained from a cross between Pusa1121 (Basmati) and MAS25 (aerobic) rice varieties for five SSR markers and BAD2 aroma gene, and (ii) carry out greenhouse evaluation F2 plants for root length & biomass, shoot length & biomass, yield per plant, 1000 grain weight, length-breadth ratio, aroma, relative water content and photosynthetic efficiency. Polymorphism for five microsatellite markers (RM144, RM234, RM302, RM339 and RM440) and BAD2 aroma gene specific (Bradbury et al. 2005) markers with a base difference of >30 bp in the amplified products, could be clearly visualized on 2.5% w/v agarose gels. Of the 60 F2 plants, 20, 18 and 22 plants, respectively had Pusa1121, MAS25 and Pusa1121 + MAS25 specific alleles at BAD2 locus. The NTSYS-PC UPGMA tree cluster as well as PCA analysis using six marker dataset grouped the 60 F2 plants and parental rice genotypes into two major groups: major group I with Pusa1121 and 18 F2 plants and major group II with MAS25 and 42 F2 plants. Cluster as well as PCA analysis clearly indicated that the F2 population was scattered between the two parental rice genotypes, Pusa1121 and MAS25, with an inclination towards MAS25. F2 population also showed wide variation for various traits including root length and/or dry biomass, yield per plant and length-breadth ratio. Yield per plant showed a significant positive correlation with fresh root weight (0.370; p=0.01) and dry root weight (0.328; p=0.01). A total of 9 – 12 plants had root length and/or biomass greater than MAS25 and nine plants had yield per plant higher than Pusa1121. A number of F2 plants were identified with higher root length and/or dry biomass, yield per plant, lengthbreadth ratio and with Pusa1121 specific alleles in homozygous or heterozygous condition at BAD2 locus; these lines shall serve as the novel material for the selection of stable aerobic Basmati rice varieties.enBacteria, Productivity, Acidity, Animal husbandry, Economic systems, Plasmids, Fermentation, Biological development, Flavouring, Organic compoundsThesis