Singh, Anup KumarPuneshwar KumarSharma, Sushil K.Soni, RavindraPorte, S.S.Saxena, R.R.Chowdhury, Tapas2022-03-242022-03-242021https://krishikosh.egranth.ac.in/handle/1/5810183794SCREENING OF BACILLUS SPECIES FOR THEIR ANTAGONISTIC POTENTIAL AGAINST FUSARIUM OXYSPORUM F. SP.CICERIS AND FIELD EVALUATION OF TWO BACILLUS SPP. FOR PLANT GROWTH PROMOTION AND FUSARIUM WILT MANAGEMENT IN CHICKPEAThe proposed investigation was carried out in Department of Agricultural Microbiology. Laboratory experiment was conducted at ICAR-National Institute of Biotic Stress Management, Baronda, Raipur and field experiment was conducted in instructional cum research farm, Collage of Agriculture, IGKV, Raipur, Chhattisgarh during 2020-21 Rabi season. The experiment was laid out in randomized block design with eighteen treatments and three replications. Fusarium wilt of chickpea is a widely distributed and the most destructive disease caused by Fusarium oxysporum f. sp. ciceri. The main objective of this study was to identify the effective Bacillus isolates against Fusarium oxysporium f.sp. ciceri under in vitro condition. Around 100 putative Bacillus isolates were isolated from 9 soil samples collected from Raipur, Bemetara and Kawardha districts of Chhattisgarh. All the isolates showed different colony morphology, cells were rod in shape and showed Gram positive reaction and two Bacillus isolates (CRS-6 and CRS-9) were collected from, ICAR-NIBSM, Baronda, Raipur. More than seventy isolates of Bacillus isolates were subjected for screening against virulent isolate of. Fusarium oxysporum f. sp. cicris and 24 were found superior in reducing the mycelial growth over control except KS-4 and RS-4. Among all isolates, the IS-10 and GS-05 isolates were found most potent against F. oxysporum f. sp. ciceris. Thirty Bacillus isolates showed anatagonism against Sclerotium rolfsii and among them namely KS-8 and BATS-08 were found most potent. Maximum mycelial growth and minimum inhibition was recorded in isolate KS-3 (71 mm and 21.11 %) followed by BKS-1 (70.5 mm and 21.66 %). Mycelial growth in control was 87.33 mm. Among the treatments, maximum germination percent 87.7% was observed in T14 (Vishal X Bacillus bingmayongensis)and minimum germination per cent 63.67 % was observed in T16 (JG-16 X Control). After, 30 DAS, we observed maximum plant height 22.8 cm in T3 (Vaibhav X Bacillus glycinifermentans ) and after 60 DAS, maximum plant height 40.13 cm in T17 (JG-16 X Bacillus bingmayongensis), After that we found maximum dry weight of plant 35.2 g in T8 (Jaki X Bacillus bingmayongensis ), At the time of maturity, dry weight was significantly higher 13.7 g, in T3 (Vaibhav X Bacillus glycinifermentans). After 30 DAS, maximum disease percent 4.9% was recorded in T1 (Vaibhav X Control) and after 60 DAS, maximum disease severity of 37.6% was recorded in T10 (RG-2009-16 X Control). After 90 DAS, maximum disease severity 80.0% was recorded in T7 (Jaki X Control). Treatment T6 (JG-226 X Bacillus glycinifermentans) yielded maximum grain 1085.83 kg ha' as compared to rest of the treatments. Primary nutrient (NPK) content was significantly affected by different treatments considered for experiment. Nitrogen uptake in grain (2.92 %) and straw (1.93 %) was found highest zin T6 (JG-226 X Bacillus glycinifermentans). Treatment T15 (Vishal X Bacillus glycinifermentans) gave significantly higher values of phosphorus content in grain (0.45 %) and straw (0.39 %) as compared to rest of the treatments. Treatment T15 (Vishal X Bacillus glycinifermentans) gave significantly higher values of potassium content in grain (0.67 %) and straw (0.96%). Inoculation of Bacillus sp improved crop growth and yield as determined by different plant growth parameters like plant height, root length, dry matter accumulation, nutrient content and control of Fusarium wilt diseases.EnglishThesis