Sharma, AbhishekSen, Bheem2022-01-122022-01-122021Sen, Bheem (2021). Detection and transmission of phytoplasma/virus(es) infecting brinjal (Solanum melongena L.) in Punjab (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.https://krishikosh.egranth.ac.in/handle/1/5810180699Phytoplasma/virus disease complex is a major obstruction against brinjal production. In present study, survey conducted in major brinjal growing areas of Punjab and observed that phytoplasma/virus disease complex was present in all surveyed areas having disease incidence ranging from 8-21 per cent causing different type of symptoms viz. yellow mosaic, mottling, wavy margin, puckering, leafroll, little leaf, giant calyx, big bud, phyllody, virescence, stunting and withes’ broom. Under Punjab conditons association of five RNA viruses viz. PepMoV, PLRV, PVX, PVYn, PVYo/c, ssDNA virus (Begomovirus) and phytoplasma (Aster yellows phytoplasma) were revealed with this disease complex. The begomoviruses associated with this disease complex were identified as Tomato leaf curl New Delhi virus and Tomato leaf curl Palampur virus. Under transmission studies whitefly was confirmed as the vector of begomoviruses. Histopathological studies of leaf lamella (showing little leaf), flower petal (showing phyllody) and giant calyx divulged many ultrastructural changes in dermal, ground and vascular tissues. In search of host plant resistance 135 cultivated genotypes and six wild relative species of brinjal were screened against phytoplasma/virus disease complex, under open field conditions. Out of these 24 cultivated genotypes and three wild species were screened against begomovirus, under artificial screening conditions. Screening results revealed that brinjal genotype P-67 and two wild species viz. Solanum sisymbrifolium and S. torvum were showing resistance against begomovirus however, in PCR assay using PALIc1960/PARIv722 primers all 24 genotypes were showing positive reaction for presence of begomovirus. But S. sisymbrifolium and S. torvum were showing negative reaction in PCR.EnglishThesis