Grewal, R.S.Bilal, Chamadia2019-10-172019-10-172019http://krishikosh.egranth.ac.in/handle/1/5810131700The present study was conducted to investigate the effect tannin on protein protection of Soybean meal on in vitro degradability, in vivo nutrient utilization, blood profile, and growth performance of buffalo heifers. The study was conducted in two phases. In the phase I SBM was treated with quebracho and tannic acid, chemical composition of treated SBM in comparison to control SBM was determined. In vitro studies were conducted using treated SBMs, and TMRs (containing treated SBM) as substrates. In Phase II, in vivo study was carried out to determine the effect of tannic acid treated SBM on nutrient utilization, performance of buffalo heifers and blood metabolites. The tannic acid lowered ammonical nitrogen significantly (P<0.05) compared to Quebracho and control. Tannic acid improved partitioning factor non-significantly (P<0.05). The TMR prepared by replacing 50% SBM with 1% tannic acid treated SBM was selected for in vivo trial on the basis of in vitro because it reduced ammonical nitrogen compared to control significantly, there was higher EMMP than control and PF was numerically higher. A 90-day growth trial was conducted on buffalo heifers. Sixteen animals were divided in to two groups. Animals were fed total mixed ration with dry roughage, non-leguminous silage and concentrate in the proportion of 10:50:40. There was no significant (P>0.05) difference between control and treatment group in total DM but CP intake was significantly (P>0.05) lower in treatment group. In vivo digestibility of CP, EE and cellulose decreased significantly (P<0.05). The tannic acid treatment of SBM gave encouraging results in terms of growth in buffalo heifers. Tannic acid significantly (P<0.05) increased weight gain/kg CP intake. It also improved FCR. Tannic acid reduced alkaline phosphatase and alanine transaminase significantly (P<0.05).ennullThesis