Banerjee, MadhuparnaMAHATO, VISHAL KUMAR2017-06-172017-06-172013http://krishikosh.egranth.ac.in/handle/1/5810021260Transformation of Finger millet (Eleusine coracana L. Gaertn.); variety A404, through Agrobacterium tumefaciensThe present research work has been carried out with a view to standardize the protocol for Agrobacterium tumefaciens mediated transformation on Eleusine coracana L. a valuable crop plant, using Agrobacterium strains GV2260 having pCAMBIA2300 plasmid. Aseptic explants for transformation were obtained by inoculating seeds of Eleusine coracana L. on MS medium supplemented with 2, 4-D 4.0 mg/L, callus was induced. Callus was inoculated on MS medium supplemented with BAP 2.0 mg/L for multiplication of shoots. For kanamycin sensitivity test of shoots showing maximum etiolation (98%), blackening (84%) and death (82%) have occurred on MS medium supplemented with 100.0 mg/L of kanamycin after 21 days of inoculation. Agrobacterium strain GV2260 was maintained at 26˚C for 20 hrs at 150 rpm in dark condition in LB medium supplemented with 50.0 mg/L rifampicin, 50.0 mg/L kanamycin and 100.0 mg/L acetocyringone. The explants were treated for different co-culture periods after which those were inoculated in solid MS medium supplemented with BAP 2.0 mg/L and kept at 25±2˚C and 16 hr light/8 hr dark. After 10 days of infection the explants were subculture on MS medium supplemented with 100.0 mg/L of kanamycin for the confirmation of transformation. After 21 days it was observed that only one explant survived out of nine explants in 30 min of coculture with Agrobacterium tumefaciens.en-USnullThesis