Mahajan, VikasMahajan, VikasMahajan, VikasAhamed, NaseerAhamed, NaseerAhamed, Naseer2022-10-072022-10-072022-09-222022-09-222022-09-22https://krishikosh.egranth.ac.in/handle/1/5810188662The aim of this study was to investigate polymorphism of growth hormone (GH) 1 gene and its association with growth traits in quail using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Blood samples of 100 quails along with their body weight at 0, 1, 2, 3, 4, 5 and 6 weeks of age were taken. Genomic DNA was extracted by using DNA kit (HIMEDIA). The amplified PCR product of GH1 gene was 776 bp in size and was digested with Msp1 restriction enzyme at 37 °C for 30 min. The resulted digested products were run on 2.5% agarose gel, showed three different genotypes: AA (539 bp, 237 bp), AB (776 bp, 539 bp, and 237 bp) and BB (776 bp). The genotypic frequencies were found to be 0.50 for the AA genotype, 0.43 for the AB genotype and 0.07 for the BB genotype, while the gene frequencies for A and B allele were 0.715 and 0.285 respectively. The population was in Hardy-Weinberg equilibrium (HWE) as evidenced by the non-significant Chi-square value. The polymorphic information content value showed moderate level of polymorphism of 0.32 that indicates the effectiveness of the marker. Least square analysis of variance revealed non-significant differences at 0, 1, 2, 3, and 4 weeks of age with respect to different genotypes of GH1 gene, whereas highly significant (P<0.01) differences were observed at 5th and 6th week of age. BB genotype has significantly (P<0.01) higher body weight followed by AB and AA genotype at 5th and 6th week of age. The results indicate that the GH1 gene may be used as a candidate gene to improve the growth traits and also selection of superior bird can be done at any early age of 5 weeks for the genetic improvement in the quail.The aim of this study was to investigate polymorphism of growth hormone (GH) 1 gene and its association with growth traits in quail using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Blood samples of 100 quails along with their body weight at 0, 1, 2, 3, 4, 5 and 6 weeks of age were taken. Genomic DNA was extracted by using DNA kit (HIMEDIA). The amplified PCR product of GH1 gene was 776 bp in size and was digested with Msp1 restriction enzyme at 37 °C for 30 min. The resulted digested products were run on 2.5% agarose gel, showed three different genotypes: AA (539 bp, 237 bp), AB (776 bp, 539 bp, and 237 bp) and BB (776 bp). The genotypic frequencies were found to be 0.50 for the AA genotype, 0.43 for the AB genotype and 0.07 for the BB genotype, while the gene frequencies for A and B allele were 0.715 and 0.285 respectively. The population was in Hardy-Weinberg equilibrium (HWE) as evidenced by the non-significant Chi-square value. The polymorphic information content value showed moderate level of polymorphism of 0.32 that indicates the effectiveness of the marker. Least square analysis of variance revealed non-significant differences at 0, 1, 2, 3, and 4 weeks of age with respect to different genotypes of GH1 gene, whereas highly significant (P<0.01) differences were observed at 5th and 6th week of age. BB genotype has significantly (P<0.01) higher body weight followed by AB and AA genotype at 5th and 6th week of age. The results indicate that the GH1 gene may be used as a candidate gene to improve the growth traits and also selection of superior bird can be done at any early age of 5 weeks for the genetic improvement in the quail.The aim of this study was to investigate polymorphism of growth hormone (GH) 1 gene and its association with growth traits in quail using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Blood samples of 100 quails along with their body weight at 0, 1, 2, 3, 4, 5 and 6 weeks of age were taken. Genomic DNA was extracted by using DNA kit (HIMEDIA). The amplified PCR product of GH1 gene was 776 bp in size and was digested with Msp1 restriction enzyme at 37 °C for 30 min. The resulted digested products were run on 2.5% agarose gel, showed three different genotypes: AA (539 bp, 237 bp), AB (776 bp, 539 bp, and 237 bp) and BB (776 bp). The genotypic frequencies were found to be 0.50 for the AA genotype, 0.43 for the AB genotype and 0.07 for the BB genotype, while the gene frequencies for A and B allele were 0.715 and 0.285 respectively. The population was in Hardy-Weinberg equilibrium (HWE) as evidenced by the non-significant Chi-square value. The polymorphic information content value showed moderate level of polymorphism of 0.32 that indicates the effectiveness of the marker. Least square analysis of variance revealed non-significant differences at 0, 1, 2, 3, and 4 weeks of age with respect to different genotypes of GH1 gene, whereas highly significant (P<0.01) differences were observed at 5th and 6th week of age. BB genotype has significantly (P<0.01) higher body weight followed by AB and AA genotype at 5th and 6th week of age. The results indicate that the GH1 gene may be used as a candidate gene to improve the growth traits and also selection of superior bird can be done at any early age of 5 weeks for the genetic improvement in the quail.EnglishThesis