SubhadraPoonam Rani2017-06-032017-06-032005http://krishikosh.egranth.ac.in/handle/1/5810014919Field experiment and isozymic analysis on 40 accessions of Sesbania were carried out with the objectives: (i) to estimate variability among accessions of Sesbania using various isozymes and morphological markers and (ii) to establish relationship among accessions using these parameters. The accessions were grown in randomized block design with three replications. The observations were recorded on 15 variables viz., plant height (at 20 and 60 days after sowing, and at maturity), green biomass, dry weight, number of root nodules, fresh weight of root nodules, leaf length, leaves per plant, leaflets per leaf, number of pods per plant , pod length, number of seeds per pod, 100-seed weight and seed yield per plant. Significant genotypic differences were observed for all the 15 morphological characters. Accession EC 493668 (T21) from Australia was identified for its direct exploitation as a cultivar. Seed yield showed positive correlation with number of pods, number of seeds per pod, pod length, leaf length and plant height at maturity. Weight of root nodules, number of pods and pod length showed high positive direct and indirect effects contribution towards their correlation with seed yield. Seed yield and its components showed medium to high heritability and genetic advance. UPGMA method with Euclidean distances based on agromorphological traits grouped accessions into seven clusters. Accessions T3 from cluster 1 and T24 from cluster 7 were recommended for hybridization programme for improvement of grain yield. Cluster 7 showed high inter cluster distance from clusters namely cluster 2, 3 and 4. The accessions were fingerprinted for 6 isozyme systems (acid phosphatase, amylase, catalase, glutamate dehydrogenase, peroxidase and malate dehydrogenase) and grouped the accessions into 11 clusters. Malate dehydrogenase and glutamate dehydrogenase exhibited maximum unique isozymic patterns i.e. 38 and 31, respectively followed by acid phosphatase, amylase, peroxidase and catalase. None of the enzyme system could alone distinguish all the accessions individually, however, a combination of any of the two enzyme systems may be used to distinguish all 40 accessions uniquely. Majority of accessions of S. rostrata were grouped together in one cluster in both agromorphological and isozymic data analysis. Both analyses indicated that geographical diversity is important but not the sole factor determining the genetic diversity.ennullThesis