Purwar, J. P.Maheshwari, Sourabh2024-08-272024-08-272023-07Theses of M.Sc.https://krishikosh.egranth.ac.in/handle/1/5810213722Nanotechnology for plant protection is an emerging research field that offers new methods to amid nanomaterials as Nanopesticide. The present study was conducted to evaluate the compatibility of AgNPs with egg Parasitoids, Trichogramma chilonis, Entomopathogenic Nematode, Steinernema abbasi strain PN-01 and Entomopathogenic fungus, Beauveria bassiana along with study on Synergistic effect of AgNPs with Beauveria bassiana alone and in combination against Spilarctia obliqua under laboratory and net house conditions. The biosynthesis of Silver nanoparticles was performed with neem leaves extract and its characterization was done with UV-Vis spectrophotometer, DLS, SEM and FTIR. The shape of the NPs was observed to be spherical and the average size was 66.63 nm. It was observed that at highest concentration of AgNPs (40 ppm), there was only 47.67 percent Adults of T. chilonis emerges from the eggs shell and 51.27 percent reduction in eggs hatching over control was recorded at 3rd day after treatment. The Probit data analysis shows that S abbasi was sensitive to AgNPs with LC50 0.37 ppb. In compatibility test, all the concentrations of AgNPs were found compatible and harmless except 20 ppm and 40 ppm for the growth of B. basssina as per Hassan’s classification index. The LC50 and LT50 values recorded under laboratory condition for combination of AgNPs and B. bassiana was 11.16 ppm and 77.31 hours, respectively against 6 days old larvae of S. obliqua. The combination was found 2.85 times more toxic than AgNPs alone. Under net house conditions, 100 percent probit mortality of S. obliqua was recorded on 8th day after treatment when combination was used, while 42.85 and 53.57 percent probit mortality was recorded for individual treatment of B. bassiana and AgNPs (40 ppm). This study reveals that AgNPs with biological control agents turn to be promising cradle of nontoxic pesticides that shows synergistic effect in controlling noxious insect pests. Further, this technique will be a valuable tool in the integrated pest management system.EnglishThesis