Hulmani, Dr. N.C.HEGDE, HEMANT G.2019-10-222019-10-222001-08-03Th-5829http://krishikosh.egranth.ac.in/handle/1/5810132432Studies were conducted to develop a protocol for micropropagation of black pepper {Piper nigrum) through axillary shoot multiplication. Also different plant propagation structures and season of planting for rooting of cuttings were evaluated. The studies were conducted at the Agricultural Research Station (Pepper), Sirsi and laboratories of the College of Forestry Sirsi, University of Agricultural Sciences, Dharwad during 1995-99. Surface disinfection of shoot tip or nodal bud explants with sodium hypochlorite (2%) for ten minutes was found optimum. The explants collected during February to May gave better establishment. The young buds from the tip to the sixth nodal segment gave better establishment of cultures. MS semisolid medium with half the strength of its inorganic salts supplemented with BA 2 mgr-1 and 30 gl-1 sucrose was suitable for shoot establishment as well as shoot multiplication (5.13 buds/culture). The multiplication rate increased with repeated subculturlng on media of same composition at 15 days interval. Rooting of in vitro derived shoots was achieved in in vitro as well as ex vitro conditions. Cent per cent rooting was obtained under in vitro conditions in medium supplemented with NAA 1 mgl-1 and 30 ql-1 sucrose. Quick dipping the cut ends of shoots in 1000 ppm ISA enhanced ex vitro rooting up to 68 per cent. Pre-hardening treatments enhanced establishment of micropropagated plantlets. A potting mixture containing 1:1 sand + coir pith was superior for establishment of plantlets. The application of 10 ml of Vi MS or Hoogland nutrient solution at weekly intervals was beneficial for vigorous growth of transplanted plantlets. The propagation of black pepper by conventional means through cuttings was best in propagation frame or polyhouse during summer (94%).ennullThesis