SUDHAKAR, K(MAJOR)VINOO, RSUBRAMANYAM, K.VSUSHMA DEVI, POSIMSETTI2018-08-022018-08-022018-01http://krishikosh.egranth.ac.in/handle/1/5810063057ABSTRACT: Bovine Tuberculosis is a chronic infectious respiratory disease caused by Mycobacterium bovis. The conventional preventive and control measures are not so effective in combating the disease, hence alternate strategies like selective breeding of animals resistant to the disease by exploiting the natural variation of the genes underlying the disease resistance trait to the disease could be considered. The present study is aimed at identifying those genes and SNPs to be associated with bTB susceptibility / resistance and exploiting the genetic variations in the gene regions by using PCR-RFLP method. Two genes (CCL4 and RNF185) were identified to be promising candidate genes in association with bTB and their SNPs that create restriction enzyme sites were considered for the present study. DNA was isolated from blood samples of 128 Ongole cattle and 155 Murrah buffaloes by modified high salt method. Two sets of primers were designed so as to capture the SNP regions of the genes. The PCR products were subjected to RFLP with BtsαI and TseI enzymes for CCL4 and RNF185 genes respectively. In the population under study, both the genes were found to be monomorphic. The fixation of allele ‘A’ is revealed in CCL4 gene with the absence of ‘T’ allele while in RNF185 gene, ‘A’ allele is fixed with absence of ‘G’ allele. Both the loci were found to be monomorphic in both cattle and buffalo.ennullThesis