Pardeep SinghSarangi, Archana2017-06-242017-06-242016http://krishikosh.egranth.ac.in/handle/1/5810023138The mammalian sperm membrane contains a large number of unsaturated fatty acids and is therefore sensitive to lipid peroxidation. Oxidative damage to spermatozoa during preservation of liquid semen at 4°C is a potential cause of the decline in motility and fertility during hypothermic storage of liquid semen. Vitamin E (DLα-Tocopherol) and glutathione are believed to be the primary component of antioxidant system of the spermatozoa. The objective of this study was to evaluate the role of vitamin E and glutathione in improving the seminal parameters during storage for 72 hours. The thirty-six semen ejaculates were collected with the help of artificial vagina from six bucks (Beetal) during the normal reproduction season. The samples were centrifuged at 1500rpm for 10 minutes and the seminal plasma was removed. The sperm pellet was diluted with Tris based extender and divided into 3 groups. Group T1 was taken as control; group T2 were supplemented with tocopherol @ 3mM and group T3 were supplemented with glutathione @ 1mM respectively. The samples were evaluated for morphological parameters viz., progressive sperm motility, percent livability, percent abnormal spermatozoa , acrosome integrity after liquid preservation for 0, 24, 48 and 72 hours and oxidative stress parameters viz., lipid peroxidation, glutathione peroxidase (GPx), superoxide dismutase (SOD) after liquid preservation for 0 and 72 hours. It was observed that after 0, 24, 48 and 72 h of storage at 4°C, the progressive sperm motility, percent livability and percent intact acrosomes were significantly (P˂0.05) higher in group T2 and T3 samples as compared to control. But, percent abnormal spermatozoa and level of lipid peroxidation in T2 and T3 group was significantly (P˂0.05) lower after 72hours of incubation at 4°C. Similarly, GPx and SOD values were highly significant (P˂0.05) in T2 and T3 group after 72 hours of storage at 4°C. Thus it can be concluded that supplementation of vitamin E and glutathione to the semen samples @ 3mM and 1mM concentration respectively, helps in maintaining the seminal parameters during liquid preservation of semen at 4°C upto72 hours.enThesis