KAUR, RAJINDERSHARMA, DEEPKA2017-01-202017-01-20200947093http://krishikosh.egranth.ac.in/handle/1/97330Abstract In the present studies on relatedness among cultivars and selected clones of almond [Prunus dulcis Miller (D. A. Webb)] using RAPD markers, DNA from fresh, young and healthy leaves of 32 almond accessions was isolated using CTAB method (Doyle and Doyle, 1987) with some modifications. Out of forty random decamer primers, only sixteen were able to amplify genomic DNA. These sixteen random decamer primers gave 87 polymorphic bands, 14 unique bands sized 100 bp to 1500 bp. The PIC value (Polymorphic Information Content) for all the sixteen primers was calculated and average was found to be 0.684. The primer S073 gave highest PIC value (0.8687) and lowest PIC value (0.2551) was obtained with primer S081. From this it was found that primer S073 was most informative. Similarity matrix using Jaccard’s coefficient was constructed and it ranged from 0.000 to 0.667. Maximum similarity was found between ‘V.1’ and ‘Tree No. 104’. Dendrogram based on UPGMA method divided the accessions into three main clusters ‘A’, ‘B’ ,‘C’ and one singlet (Nauni Selection) was obtained at 0.02 similarity index value. Cophenetic correlation was found to be 0.89. From all the data obtained it can be concluded that polymorphism can be easily scored and used for studying relatedness almond genotypes using RAPD markers.enThesis