Priya, Y.Senthilkumar, T.M.A.Mangalagowri, A.TANUVAS2019-09-062019-09-062013http://krishikosh.egranth.ac.in/handle/1/5810126687TNV_TH_2013_MPB12001Gene therapy is a modern medicine in the present decade to treat many diseases. It is promising a great future in saving many lives in the present world. Based on this, we developed chitosan nanoparticles which can be used as vehicle to transfect the plasmid DNA pEGFP-C1 expressing green fluorescence in the host cells. It was prepared by ionic gelation with polyanions to couple with plasmid and transfect the plasmid into host cells.en-USVeterinary ScienceAnimal BiotechnologyThesis