Satbige, S. AjayVijaya Bharathi, M.Ganesan, P.I.Sreekumar, C.TANUVAS2017-11-282017-11-282016-02http://krishikosh.egranth.ac.in/handle/1/5810036563TNV_Com_Abs_Feb2016_17-19_Pg53A total of 193 sera samples, along with tissues (lung, heart, and brain] collected from 136 sheep and 57 goats from the Corporation slaughter house, Madras Veterinary College teaching hospital, and private mutton shops from Chennai were tested for Toxoplasma gondii. All the sera samples were tested using modified direct agglutination test (MAT). Of the 193 sera samples, 57 (29.5 per cent) had a minimum titre of 1:20. Of 57 samples, 30.14 per cent (41/136) ofsheep and 28.07 per cent (16/57) of goats being seropositive. Tissue samples from all 193 animals were subjected to B1 based PCR to detect T gondii DNA showed 3.67 per cent and 3.50 per cent to be positive in sheep and goats, respectively. in the present investigation B1 based PCR detected T.‘ gondii in low numbers, possibly due to limitation of the sample size. The presence of Tgondii in tissues of sheep and goats slaughtered for human consumption in Chennai indicates the role of these food animals as potential sources of infection to human.enVeterinary MedicineOther