Dr.R. S. FougatAmit Kumar2019-06-142019-06-142016http://krishikosh.egranth.ac.in/handle/1/5810108458The endogenous small non-coding functional microRNAs (miRNAs) are short in size, ranging from ~21 to 24 nucleotides in length and play a pivotal role in gene expression in plants and animals by negative regulation or silencing of genes either by cleavage or blocking of translation of homologous mRNA. Although various high throughput and expensive techniques like forward genetics and direct cloning are employed to detect miRNAs in plants but comparative genomics complemented with novel bioinformatic tools paced the way for efficient and cost-effective identification of miRNAs through homologous sequence search with previously known miRNAs.en-USnullThesis